The complete sequence of soybean chlorotic mottle virus DNA and the identification of a novel promoter.
The complete sequence of soybean chlorotic mottle virus DNA and the identification of a novel promoter.
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DOI：
10.1093/nar/17.23.9993
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发表时间：
1989-12
影响因子：
14.9
通讯作者：
Akira Hasegawa;J. Verver;A. Shimada;Miho Saito;R. Goldbach;A. Kammen;K. Miki;M. Kameya-Iwaki;T. Hibi
中科院分区：
文献类型：
--
作者：
Akira Hasegawa;J. Verver;A. Shimada;Miho Saito;R. Goldbach;A. Kammen;K. Miki;M. Kameya-Iwaki;T. Hibi
测定了大豆褪绿斑驳病毒（soybean chlorotic mottle virus，CMV）侵染性克隆的全序列，并与花椰菜花叶病毒（cauliflower mosaic virus，CaMV）、康乃馨蚀环病毒（carnation etched ring virus）和玄参花叶病毒（figwort mosaic virus）的全序列进行了比较。双链DNA基因组（8，175 bp）含有9个开放阅读框（ORF）和一个较大的基因间区。ORF的引物结合位点、基因结构和大小与其他花椰菜花叶病毒相似，但ORF I分为ORF Ia和ORF Ib。从每个ORF推导的氨基酸序列显示只有短的，高度同源的区域中的几个相应的ORF的其他三个花椰菜花叶病毒。一个启动子片段的378 bp的在烟草CMV ORF III显示出较强的表达活性，相当于的CaMV 35 S启动子，在烟草叶肉原生质体通过β-葡萄糖醛酸酶测定使用电转染。该片段含有CAAT和TATA盒，但没有CaMV 35 S启动子所报道的转录增强子信号。相反，它具有与烟草花叶病毒RNA的5 '-前导序列报道的翻译增强子信号的一部分同源的序列。
The complete nucleotide sequence of an infectious clone of soybean chlorotic mottle virus (SoyCMV) DNA was determined and compared with those of three other caulimoviruses, cauliflower mosaic virus (CaMV), carnation etched ring virus and figwort mosaic virus. The double-stranded DNA genome of SoyCMV (8,175 bp) contained nine open reading frames (ORFs) and one large intergenic region. The primer binding sites, gene organization and size of ORFs were similar to those of the other caulimoviruses, except for ORF I, which was split into ORF Ia and Ib. The amino acid sequences deduced from each ORF showed only short, highly homologous regions in several of the corresponding ORFs of the three other caulimoviruses. A promoter fragment of 378 bp in SoyCMV ORF III showed a strong expression activity, comparable to that of the CaMV 35S promoter, in tobacco mesophyll protoplasts as determined by a beta-glucuronidase assay using electrotransfection. The fragment contained CAAT and TATA boxes but no transcriptional enhancer signal as reported for the CaMV 35S promoter. Instead, it had sequences homologous to a part of the translational enhancer signal reported for the 5'-leader sequence of tobacco mosaic virus RNA.