Functional analysis of MADS-box genes controlling ovule development in Arabidopsis using the ethanol-inducible alc gene-expression system
Functional analysis of MADS-box genes controlling ovule development in Arabidopsis using the ethanol-inducible alc gene-expression system
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DOI：
10.1016/j.mod.2006.01.002
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发表时间：
2006-04-01
影响因子：
2.6
通讯作者：
Kater, Martin M.
中科院分区：
文献类型：
--
作者：
Battaglia, Raffaella;Brambilla, Vittoria;Kater, Martin M.
在拟南芥中，不同组合的ABC器官身份蛋白在SEPALLATA（SEP）蛋白的存在下相互作用，以调节花器官分化。SEP 3与A类和B类或B类和C类基因的组合的异位表达足以将营养叶同源异型地转化为花瓣样器官并且将苞片同源异型地转化为雄蕊样结构。分别最近，已经表明三个MADS盒基因SEEDSTICK（STK）、SHATTERPROOF 1（SHP 1）和SHP 2冗余地控制胚珠身份。在酵母中进行的蛋白质相互作用试验结合遗传研究表明，这些MADS盒因子只有在SEP蛋白的存在下相互作用，形成复合物，确定胚珠分化。在这里，我们解决的问题是否异位共表达胚珠身份蛋白是足以诱导同源异型转化的营养叶心皮样结构轴承胚珠。我们提出了拟南芥植物的表型特征，异位表达胚珠身份因子的调控下，乙醇诱导的基因表达系统。这些实验表明SEP 3和SHP 1和/或STK的异位共表达可能不足以将营养组织同源转化为具有胚珠的心皮。然而，比较通过STK和/或SHP 1在有或没有SEP 3的情况下异位表达获得的表型显示，能够在酵母中形成复合物的因子的共表达引起更极端的同源异型转化，证实了这些复合物在体内的功能作用。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
In Arabidopsis, different combinations of ABC organ identity proteins interact in the presence of SEPALLATA (SEP) proteins to regulate floral organ differentiation. Ectopic expression of SEP3 in combination with class A and B or B and C genes is sufficient to homeotically convert vegetative leaves into petal-like organs and bracts into stamen-like structures. respectively. Recently, it has been shown that the three MADS-box genes SEEDSTICK (STK), SHATTERPROOF1 (SHP1) and SHP2 act redundantly to control ovule identity. Protein interaction assays performed in yeast in combination with genetic Studies demonstrated that these MADS-box factors only interact in the presence of SEP proteins to form complexes that determine ovule differentiation. Here, we address the question whether the ectopic co-expression of ovule identity proteins is sufficient to induce the homeotic conversion of vegetative leaves into carpel-like structures bearing ovules. We present the phenotypic characterization of Arabidopsis plants that ectopically express ovule identity factors under the regulation of the ethanol inducible gene expression system. These experiments indicate that the ectopic co-expression of SEP3 and SHP1 and/or STK is probably not sufficient to homeotically transform vegetative tissues into carpels with ovules. However, comparing the phenotypes obtained by ectopic expression of STK and/or SHP1 with or without SEP3 shows that co-expression of factors that are able to form complexes in yeast cause more extreme homeotic transformations, confirming the functional role of these complexes in vivo. (c) 2006 Elsevier Ireland Ltd. All rights reserved.