Elevated levels of the receptor for advanced glycation end products, a marker of alveolar epithelial type I cell injury, predict impaired alveolar fluid clearance in isolated perfused human lungs.
Elevated levels of the receptor for advanced glycation end products, a marker of alveolar epithelial type I cell injury, predict impaired alveolar fluid clearance in isolated perfused human lungs.
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DOI：
10.1378/chest.08-0919
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发表时间：
2009-02
期刊：
影响因子：
9.6
通讯作者：
Matthay MA
中科院分区：
文献类型：
--
作者：
Briot R;Frank JA;Uchida T;Lee JW;Calfee CS;Matthay MA
虽然肺泡上皮损伤是急性肺损伤患者预后的主要决定因素，但目前尚无可靠的肺泡上皮损伤生物学标志物。主要目的是确定晚期糖基化终末产物（AGEs）受体（肺泡上皮损伤的标志物）水平升高是否反映了离体灌注人肺制剂中肺泡液体清除率（AFC）受损。第二个目的是确定血管性血友病因子抗原（vWF：Ag）内皮损伤标志物的水平是否与受损的AFC相关。在12 mm Hg的恒定肺动脉压下灌注由加州移植供体网络拒绝移植的人肺（N = 30）。复温至36°C后，用10 cm H2O的持续气道正压对肺进行充气。然后测定血清TNF-α和vWF：Ag水平及AFC率。AFC发生率与肺泡液中TNF水平呈负相关（p < 0.005）。同样，亚最大AFC肺（在预先规定的分析中定义为≤ 14%/h）的肺泡液中的AUC浓度显著高于AFC保留肺（中位数0.82 vs 0.43 μg/mL; p < 0.05）。与此相反，vWF：Ag水平与AFC的发生率无关。在移植前供体肺和急性肺损伤患者中，肺泡上皮损伤和AFC受损可能是一个有用的生物学标志物。
Although alveolar epithelial injury is a major determinant of outcome in patients with acute lung injury, there is no reliable biological marker of alveolar epithelial injury. The primary objective was to determine whether elevated levels of the receptor for advanced glycation end products (RAGE), a marker of alveolar epithelial injury, reflect impaired alveolar fluid clearance (AFC) in an ex vivo perfused human lung preparation. A second objective was to determine whether levels of a marker of endothelial injury, von Willebrand factor antigen (vWF:Ag), are associated with impaired AFC. Human lungs (N = 30) declined for transplantation by the California Transplant Donor Network were perfused at a constant pulmonary artery pressure of 12 mm Hg. Following rewarming to 36°C, the lungs were inflated with a continuous positive airway pressure of 10 cm H2O. RAGE and vWF:Ag levels and AFC rates were then measured. The rate of AFC was inversely correlated with RAGE levels in the alveolar fluid (p < 0.005). Similarly, the concentration of RAGE in the alveolar fluid was significantly higher in lungs with submaximal AFC, defined in a prespecified analysis as ≤ 14%/h, when compared with lungs with preserved AFC (median 0.82 vs 0.43 μg/mL; p < 0.05). In contrast, vWF:Ag levels did not correlate with the rate of AFC. RAGE may be a useful biological marker of alveolar epithelial injury and impaired AFC in donor lungs prior to transplant and perhaps in patients with acute lung injury.
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影响因子：
15.9
作者：
RUBIN, DB;WIENERKRONISH, JP;MATTHAY, MA
通讯作者：
MATTHAY, MA
影响因子：
8.9
作者：
de Antonio, David Gomez;Marcos, Roberto;Varela, Andres
通讯作者：
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影响因子：
8.9
作者：
Fiser, SM;Kron, IL;Tribble, CG
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Tribble, CG
影响因子：
168.9
作者：
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通讯作者：
Algotsson, L
影响因子：
4.6
作者：
Steen, Stig;Ingemansson, Richard;Sjoberg, Trygve
通讯作者：
Sjoberg, Trygve