Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury.
Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury.
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凝溶胶蛋白：功能蛋白在减轻辐射损伤中的作用。
DOI：
10.1007/s12013-014-0210-3
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
rui min
中科院分区：
文献类型：
--
作者：
ming-juan li;feng-mei cui;ying cheng;ling han;jia wang;ding sun;yu-long liu;ping-kun zhou;rui min
本研究旨在探讨外源性凝溶胶蛋白（GSN）对大剂量辐射小鼠的防护作用。以固定剂量率的137Cs γ射线照射小鼠，观察小鼠外周血和培养的人肠上皮细胞（HIECs）胞浆中GSN含量的变化。测定凝血相关指标：凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）。通过评估血液中谷胱甘肽（GSH）和丙二醛（MDA）浓度的变化来评价对辐射介导的氧化损伤的影响。结果表明，辐射诱导pGSN血液水平显著降低。然而，辐照HIECs的cGSN水平以剂量依赖性方式增加。重组人pGSN给药照射小鼠导致凝血时间改善，如PT和APTT指数所示。用hpGSN治疗小鼠提高了GSH的血液水平，而MDA浓度降低，表明抗氧化状态改善。这些结果表明，GSNs可能在抑制急性辐射引起的组织损伤中发挥调节作用。
The present study was conducted to explore the protective effect of exogenous gelsolin (GSN) in mice exposed to high-dose of radiation. Changes in the levels of GSNs in peripheral blood of mice and cytoplasm of cultured human intestinal epithelial cells (HIECs) were analyzed after their exposure to different doses of137Cs γ-rays at a fixed dose rate. The coagulation associated indices, such as prothrombin time (PT) and activated partial thromboplastin time (APTT) were measured. Effect on radiation-mediated oxidative damage was evaluated by estimating the altered glutathione (GSH) and malondialdehyde (MDA) concentrations in the blood. The results showed that radiation induced a pronounced decrease in the pGSN blood levels. However, the cGSN levels of irradiated HIECs were increased in a dose-dependent manner. Administration of recombinant human pGSN to irradiated mice resulted in an ameliorated clotting time as indicated by the PT and the APTT indices. The treatment of mice with hpGSN enhanced the blood levels of GSH while MDA concentrations were decreased indicating an improved antioxidant status. These results suggest that GSNs might play a regulatory role in the suppression of the tissue damage induced by acute radiation exposure.