Effects of bowel preparation on the human gut microbiome and metabolome
Effects of bowel preparation on the human gut microbiome and metabolome
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DOI：
10.1038/s41598-019-40182-9
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发表时间：
2019-03-11
影响因子：
4.6
通讯作者：
Hattori, Masahira
中科院分区：
文献类型：
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作者：
Negata, Naoyoshi;Tohya, Mari;Hattori, Masahira
大肠准备可能导致肠道微生物群和代谢物的实质性变化。在这里，我们包括肠道准备组和非手术对照组，并评估了肠道准备对肠道微生物组和代谢组稳定性以及恢复的影响。分别通过16S rRNA测序和毛细管电泳飞行时间质谱分析肠道微生物群和代谢组组成。在属和物种水平上的系数和加权UniFrac距离的分析表明，与对照相比，微生物群组成在制备后立即显著减少，但在制备后14天没有显著减少。术前与术后即刻的相关系数显著低于术前与术后14天的相关系数，与受试者之间差异的比较。32种代谢物在制备前和制备后立即发生显著变化，但这些代谢物在14天内恢复。总之，肠道准备对肠道微生物组和代谢组有深远的影响，但总体组成在14天内恢复到基线。为了正确进行人类肠道微生物组和代谢组的研究，应避免在肠道准备后立即进行粪便采样。
Large bowel preparation may cause a substantial change in the gut microbiota and metabolites. Here, we included a bowel prep group and a no-procedure control group and evaluated the effects of bowel prep on the stability of the gut microbiome and metabolome as well as on recovery. Gut microbiota and metabolome compositions were analyzed by 16S rRNA sequencing and capillary electrophoresis time-of-flight mass spectrometry, respectively. Analysis of coefficients at the genus and species level and weighted UniFrac distance showed that, compared with controls, microbiota composition was significantly reduced immediately after the prep but not at 14 days after it. For the gut metabolome profiles, correlation coefficients between before and immediately after the prep were significantly lower than those between before and 14 days after prep and were not significantly different compared with those for between-subject differences. Thirty-two metabolites were significantly changed before and immediately after the prep, but these metabolites recovered within 14 days. In conclusion, bowel preparation has a profound effect on the gut microbiome and metabolome, but the overall composition recovers to baseline within 14 days. To properly conduct studies of the human gut microbiome and metabolome, fecal sampling should be avoided immediately after bowel prep.