FLOW-INJECTION ENZYME-IMMUNOASSAY OF HAPTENS WITH ENHANCED CHEMILUMINESCENCE DETECTION
FLOW-INJECTION ENZYME-IMMUNOASSAY OF HAPTENS WITH ENHANCED CHEMILUMINESCENCE DETECTION
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DOI：
10.1016/s0003-2670(00)83930-6
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发表时间：
1990-10-15
影响因子：
6.2
通讯作者：
EGOROV, AM
中科院分区：
文献类型：
--
作者：
AREFYEV, AA;VLASENKO, SB;EGOROV, AM
本文描述了一种用于甲状腺素（半抗原模型）的非平衡流动注射酶免疫分析方法，该方法包括辣根过氧化物酶标记抗体和抗原的免疫复合物与游离标记抗体的亲和分离。采用增强化学发光反应检测辣根过氧化物酶作为抗体标记物。该方法允许在5分钟内测定低至10-11 M的甲状腺素浓度，精密度为10%（相对标准偏差）。
A non-equilibrium flow-injection enzyme immunoassay for thyroxine (model hapten) involving affinity separation of the immunocomplex of horseradish peroxidase-labelled antibodies and antigen from the free labelled antibodies is described. An enhanced chemiluminescence reaction was used for detection of horseradish peroxidase as an antibody marker. The method allows the determination of thyroxine concentrations as low as 10-11 M within 5 min and the precision is 10% (relative standard deviation).