Simultaneous Increase in Multiple Proinflammatory Cytokines in the Aqueous Humor in Neovascular Glaucoma With and Without Intravitreal Bevacizumab Injection
Simultaneous Increase in Multiple Proinflammatory Cytokines in the Aqueous Humor in Neovascular Glaucoma With and Without Intravitreal Bevacizumab Injection
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DOI：
10.1167/iovs.14-15918
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发表时间：
2015-06-01
影响因子：
4.4
通讯作者：
Tanihara, Hidenobu
中科院分区：
文献类型：
--
作者：
Ohira, Saori;Inoue, Toshihiro;Tanihara, Hidenobu
目的.目的探讨新生血管性青光眼（NVG）患者房水中促炎细胞因子的水平，并分析背景因素对这些分子表达的影响。这项横断面研究招募了137名参与者，他们被分为（1）原发性开角型青光眼（POAG; n = 36）患者;（2）NVG患者（NVG; n = 33）;和（3）白内障手术患者作为对照组（CG; n = 68）。在手术开始时从前房收集房水样品，将其存放在CryoTubes中，登记并储存在-80 ° C下直至处理。使用xMAP和人细胞因子/趋化因子组I进行基于多重微粒的免疫检测。22例NVG患者术前1 ~ 2d玻璃体腔内注射贝伐单抗（IVB组），11例NVG患者术前3个月未接受抗血管内皮生长因子（VEGF）治疗（N组）。两个变量的Wilcoxon秩和检验或Fisher精确检验和多个变量的Tukey-Kramer诚实显著差异检验用于比较细胞因子水平。与CG组和POAG组相比，NVG组患者的白细胞介素（IL）-6，IL-8，单核细胞趋化蛋白（MCP）-1，肿瘤坏死因子-α（TNF-α）和血小板衍生生长因子（PDGF）AA水平较高。IVB组IL-6、IL-8、MCP-1和PDGF-AB/BB水平均高于N组，而VEGF水平则低于N组（P < 0.01）。玻璃体内注射贝伐单抗可降低VEGF水平，但对其他细胞因子无影响。
PURPOSE. To investigate aqueous humor proinflammatory cytokine levels of patients with neovascular glaucoma (NVG), and to analyze the effects of background factors in the expression of these molecules.METHODS. This cross-sectional study enrolled 137 participants who were grouped into (1) primary open-angle glaucoma (POAG; n = 36) patients; (2) NVG patients (NVG; n = 33); and (3) cataract surgery patients as a comparative group (CG; n = 68). Aqueous humor samples were collected from the anterior chamber at the start of surgery, deposited in CryoTubes, registered, and stored at -80 degrees C until processing. Multiplex microparticle-based immunodetection was performed by using xMAP and the Human Cytokine/Chemokine Panel I. Bevacizumab was injected into the vitreous cavity 1 to 2 days before surgery in 22 NVG patients (IVB group), whereas 11 NVG patients received no antivascular endothelial growth factor (VEGF) therapy 3 months preoperatively (N group). The Wilcoxon rank sum test or Fisher's exact test for two variables and the Tukey-Kramer honestly significant difference test for multiple variables were used to compare the cytokine levels.RESULTS. The NVG patients had higher levels of interleukin (IL)-6, IL-8, monocyte chemotactic protein (MCP)-1, tumor necrosis factor-alpha (TNF-alpha), and platelet-derived growth factor (PDGF)AA compared to both the CG and POAG groups. The levels of IL-6, IL-8, MCP-1, and PDGF-AB/BB were higher in the IVB group than the N group, whereas the VEGF level was significantly lower in the IVB group (P < 0.01).CONCLUSIONS. Intravitreal bevacizumab injection decreased VEGF levels, but not those of the other cytokines.