Neuronal growth factor regulation of two different sodium channel types through distinct signal transduction pathways.
Neuronal growth factor regulation of two different sodium channel types through distinct signal transduction pathways.
复制标题
神经元生长因子通过不同的信号转导途径调节两种不同的钠通道类型。
DOI：
10.1083/jcb.122.4.915
复制
发表时间：
1993-08
影响因子：
7.8
通讯作者：
Mandel, G
中科院分区：
文献类型：
--
作者：
D'Arcangelo, G;Paradiso, K;Shepherd, D;Brehm, P;Halegoua, S;Mandel, G
神经生长因子调节分化的交感神经元和PC 12细胞电压激活钠电流的表达。我们发现，在PC 12细胞中，NGF和FGF诱导的钠电流的结果从两个不同的钠通道类型的表达增加。钠电流是由一种新的钠通道转录本的快速诱导和脑II/IIA型mRNA的长期诱导引起的，该转录本也存在于外周神经元中。II/IIA型钠通道的表达需要激活环AMP依赖性蛋白激酶（A-激酶），而外周神经元型钠通道的诱导通过A-激酶非依赖性信号转导途径发生。这些发现表明，这两种钠通道类型协同作用，以确保在神经元分化过程中产生动作电位。
Neuronal growth factors regulate the expression of voltage-activated sodium current in differentiating sympathetic neurons and PC12 cells. We show that, in PC12 cells, the NGF- and FGF-induced sodium current results from increased expression of two distinct sodium channel types. Sodium current results from the rapid induction of a novel sodium channel transcript, also found in peripheral neurons, and from the long term induction of brain type II/IIA mRNA. Expression of the type II/IIA sodium channel requires activation of the cyclic AMP-dependent protein kinase (A-kinase), whereas induction of the peripheral neuron type sodium channel occurs through an A-kinase-independent signal transduction pathway. These findings suggest that the two sodium channel types act in concert to ensure the generation of action potentials during neuronal differentiation.