Surface enhanced Raman scattering study of horse heart cytochrome c at a silver electrode in the presence of bis(4-pyridyl)disulfide and purine
Surface enhanced Raman scattering study of horse heart cytochrome c at a silver electrode in the presence of bis(4-pyridyl)disulfide and purine
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双(4-吡啶基)二硫化物和嘌呤存在下银电极上马心细胞色素 c 的表面增强拉曼散射研究
DOI：
10.1016/s0022-0728(84)80371-x
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发表时间：
1984
影响因子：
4.5
通讯作者：
K. Yasukouchi
中科院分区：
文献类型：
--
作者：
I. Taniguchi;M. Iseki;H. Yamaguchi;K. Yasukouchi
电极和c型细胞色素之间直接、快速的电子转移一直是当前生物电分析化学领域的研究热点。在Eddowes和Hill发现的第一个启动子4,4′-联吡啶的存在下，观察到细胞色素c在金[3—6]和铂[7]电极上的非均相电子转移速率的加速。最近，我们已经证明，双（4-吡啶基）二硫化物（PySSPy）修饰的金电极可以作为细胞色素c快速电子转移的合适电极，在溶液中没有任何启动子或介质，尽管PySSPy本身在感兴趣的电位下是电非活性的。最近，我们报道了嘌呤及其衍生物也是有效的细胞色素c[9]的新启动子。然而，电子转移动力学增强的原因尚不清楚。Hill和同事[3- 6]认为细胞色素c的电子转移发生在启动子吸附的导电层上，而Hinnen等人[[10]]从光谱反射测量的结果来看，细胞色素c和4,4 '-联吡啶一起吸附在金电极上，细胞色素c和启动子之间的强相互作用值得怀疑。最近，Cotton等人报道，通过表面增强拉曼散射（SERS）测量，Ag (I)—4,4′-联吡啶配合物在Ag电极上形成，促进了细胞色素c在电极上的电子转移。然而，迄今为止，对促进剂修饰电极的表面轮廓知之甚少。为了更好地理解细胞色素c与启动子修饰电极之间的相互作用，有必要澄清启动子吸附电极表面的结构。出于这个目的，SERS测量是最强大的技术之一。事实上，细胞色素c在PySSPy和嘌呤存在下的SERS光谱提供了电极上吸附物质的有趣信息。
Direct, rapid electron-transfer between electrodes and c-type cytochromes has been the subject of current interest in bioelectroanalytical chemistry [1]. In the presence of 4, 4'-bipyridine, which was the first promoter found by Eddowes and Hill [2], the acceleration of the rate of heterogeneous electrontransfer of cytochrome c at gold [3--6] and platinum [7] electrodes has been observed. Recently, we have shown [8] that a bis (4-pyridyl) disulfide (PySSPy) modified gold electrode works as a suitable electrode for rapid electron-transfer of cytochrome c without any promoter or mediator in the solution, although PySSPy itself is electro-inactive at the potentials of interest. More recently, we have reported that purine and some of its derivatives are also effective as new promoters for cytochrome c [9].However, the origin of the enhancement of electron-transfer kinetics is not yet well understood. Hill and co-workers [3--6] suggested that the electrontransfer of cytochrome c takes place on the promoter-adsorbed conducting layer, while Hinnen et al.[10] claimed, from the results of spectroreflectance measurement, that cytochrome c and 4, 4'-bipyridine are adsorbed together on a gold electrode and the strong interaction between cytochrome c and the promoter is questionable. Very recently, Cotton et al.[11] have reported, by surface enhanced Raman scattering (SERS) measurement, that an Ag (I)--4, 4'-bipyridine complex is formed on the Ag electrode which facilitates the electron-transfer of cytochrome c at the electrode. However, to date, little is known about the surface profile of a promoter-modified electrode. To understand the interaction between cytochrome c and a promoter-modified electrode better, it is essential to clarify the structure of the promoter-adsorbed surface of the electrode. For this purpose, the SERS measurement is one of the most powerful techniques. In fact, SERS spectra of cytochrome c in the presence of PySSPy and purine provided interesting information about the adsorbed species on the electrode.