Gracilibacillus quinghaiensis sp nov., isolated from salt-lake sediment in the Qaidam Basin, north-west China
Gracilibacillus quinghaiensis sp nov., isolated from salt-lake sediment in the Qaidam Basin, north-west China
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青海纤细杆菌
DOI：
10.1016/j.syapm.2008.05.001
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发表时间：
2008-08-01
影响因子：
3.4
通讯作者：
Jiang, Cheng-Lin
中科院分区：
文献类型：
--
作者：
Chen, Yi-Guang;Cui, Xiao-Long;Jiang, Cheng-Lin
有活力，革兰氏阳性。微嗜盐，内生孢子形成。过氧化氢酶和氧化酶阳性、专性需氧、细长杆状细菌，菌株YIM-C229(T)是从柴达木盆地盐湖沉积物中分离得到的。中国西北部。丝状形式存在于整个生长周期。菌株YIM-C229(T)在0.5-8% NaCl存在和pH 6.0-8.5下生长，在1-3% NaCl和pH 7.0-7.5下生长最佳。它的生长温度为4-45℃，最佳生长温度为37℃。主要的细胞脂肪酸是anteiso C-15:0、C-16:0、iso-C-15:0、C-16:1 omega 11c和anteiso-C-17:0。主要的呼吸醌是MK-7，二磷脂酰甘油和磷脂酰甘油是极性脂质，内消旋二氨基庚二酸存在于细胞壁肽聚糖中。基因组DNA G+C含量为40.9mol%。基于16S rRNA基因序列的系统发育分析表明，菌株YIM-C229(T)与Gracilibacillus属4个已识别种的模式菌株：G. halotolerans NNT（序列相似性95.5%）、G. dipsosauri DD1（T）（96.1%）、G. orientalis XH-63（T）（96.8%）和G. orientalis XH-63（T）（96.8%）密切相关。 G. boraciitolerans T-16X(T) (99.1%)。菌株YIM-C229(T)与G. boraciitolerans DSM 17256(T)之间的DNA-DNA相关性为30.8%。系统发育分析、表型特征、化学分类学差异和DNA-DNA杂交数据的结合支持了该菌株代表Gracilibacillus属的新种的观点，其名称为Gracilibacillus quinghaiensis sp。十一月提出，以 YIM-C229(T) (= DSM 17858(T) = CGMCC 1.6304(T)) 作为类型菌株。 (c) 2008 年爱思唯尔有限公司。版权所有。
A motile, Gram-positive. slightly halophilic, endospore-forming. catalase- and oxidase-positive, obligately aerobic, slender rod-shaped bacterium, strain YIM-C229(T) was isolated from the sediment of a salt lake in the Qaidam Basin. north-west China. Filamentous forms were present throughout the growth cycle. Strain YIM-C229(T) grew in the presence of 0.5-8% NaCl and at pH 6.0-8.5, with optimum growth at 1-3% NaCl and pH 7.0-7.5. It grew at 4-45 degrees C, with optimum growth at 37 degrees C. The major cellular fatty acids were anteiso C-15:0, C-16:0, iso-C-15:0, C-16:1 omega 11c and anteiso-C-17:0. The predominant respiratory quinone was MK-7, and diphosphatidylglycerol and phosphatidyl-glycerol were the polar lipids, with meso-diaminopimelic acid occurring in the cell-wall peptidoglycan. The genomic DNA G + C content was 40.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM-C229(T) was closely related to the type strains of the four recognized species of the genus Gracilibacillus: G. halotolerans NNT (sequence similarity 95.5%), G. dipsosauri DD1(T) (96.1%), G. orientalis XH-63(T) (96.8%) and G. boraciitolerans T-16X(T) (99.1%). The DNA-DNA relatedness between strain YIM-C229(T) and G. boraciitolerans DSM 17256(T) was 30.8%. The combination of phylogenetic analysis, phenotypic characteristics, chemotaxonomic differences and DNA-DNA hybridization data supported the view that this strain represented a novel species of the genus Gracilibacillus, for which the name Gracilibacillus quinghaiensis sp. nov. is proposed, with YIM-C229(T) (= DSM 17858(T) = CGMCC 1.6304(T)) as the type strain. (c) 2008 Elsevier GmbH. All rights reserved.