PRODUCTION OF LATENT TRANSFORMING GROWTH-FACTOR-BETA AND OTHER INHIBITORY FACTORS BY CULTURED MURINE IRIS AND CILIARY BODY CELLS
PRODUCTION OF LATENT TRANSFORMING GROWTH-FACTOR-BETA AND OTHER INHIBITORY FACTORS BY CULTURED MURINE IRIS AND CILIARY BODY CELLS
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DOI：
10.3109/02713689109013870
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发表时间：
1991-08-01
影响因子：
2
通讯作者：
GRANSTEIN, RD
中科院分区：
文献类型：
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作者：
KNISELY, TL;BLEICHER, PA;GRANSTEIN, RD
房水含有转化生长因子-β（TGF-β）和其他细胞增殖抑制因子。在本研究中，我们调查了这些因素是由虹膜和睫状体内发现的细胞局部产生的可能性。从C57 BL/6小鼠的虹膜和睫状体（I/C-B）细胞或整个组织外植体产生的可溶性因子抑制小鼠胸腺细胞和水貂肺上皮细胞增殖。吲哚美辛部分阻断胸腺细胞增殖的抑制，但不影响Mv 1 Lu增殖的抑制。培养上清液的抑制活性不被TGF-β-1或TCF-β-2的中和抗体阻断。然而，在来自I/CB和角膜组织的培养物上清液的酸活化之后，检测到与潜伏性TGF-β活化一致的抑制活性增加。抗体中和实验表明，这种活性的增加主要是由于I/CB组织的TGF-β-2。从I/CB组织mRNA产生的cDNA的聚合酶链反应（PCR）分析显示代表TGF-β-1和TGF-β-2 mRNA的突出片段。然而，角膜组织显示TGF-β-1 mRNA的突出片段，但TGF-β-2 mRNA的条带或几乎检测不到。因此，仍然不确定TGF-β-2 mRNA是否由该菌株的角膜产生。总体而言，这些结果表明，三种不同类别的抑制增殖的物质可能由常驻虹膜和睫状体细胞局部产生：1）吲哚美辛敏感产物，2）潜伏形式的TGF-β-2，3）不被吲哚美辛或抗TGF-β中和抗体阻断的因子。由这些组织产生的产物可能在对引入前房的抗原的免疫应答的发展中具有重要的调节特性。
Aqueous humor contains transforming growth factor-beta (TGF-beta) and other inhibitory factors for cellular proliferation. In the present study we investigated the possibility that these factors are produced locally by cells found within the iris and ciliary body. Iris and ciliary body (I/C-B) cells or whole tissue explants from C57BL/6 mice produced soluble factors which inhibited both murine thymocyte and mink lung epithelial cell proliferation. Indomethacin partially blocked inhibition of thymocyte proliferation, but did not affect inhibition of Mv1 Lu proliferation. The inhibitory activity of culture supernatants was not blocked by neutralizing antibodies to TGF-beta-1 or TCF-beta-2. However, following acid activation of culture supernatants from both I/CB and corneal tissue, increased inhibitory activity consistent with activation of latent TGB-beta was detected. Antibody neutralization experiments demonstrated that this increase in activity was due primarily to TGF-beta-2 for I/CB tissue. Polymerase chain reaction (PCR) analysis of cDNA generated from I/CB tissue mRNA showed prominent fragments representing both TGF-beta-1 and TGF-beta-2 mRNA. Corneal tissue, however, showed a prominent fragment for TGF-beta-1 mRNA, but either no band or a barely detectable fragment for TGF-beta-2 mRNA. Therefore, it remains uncertain whether TGF-beta-2 mRNA is produced by the cornea in this strain. Overall, these results demonstrated that three distinct categories of substances inhibitory to proliferation may be locally produced by resident iris and ciliary body cells: 1) indomethacin sensitive products, 2) TGF-beta-2 in latent form, and 3) factors not blocked by indomethacin or anti-TGF-beta neutralizing antibodies. Products generated by these tissues may have important regulatory properties in the development of immune responses to antigens introduced into the anterior chamber.