Uptake of fluorescence-labeled dextrans by 10T 1/2 fibroblasts following permeation by rectangular and exponential-decay electric field pulses.
Uptake of fluorescence-labeled dextrans by 10T 1/2 fibroblasts following permeation by rectangular and exponential-decay electric field pulses.
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发表时间：
1988-06
期刊：
影响因子：
2.7
通讯作者：
H. Liang;W. J. Purucker;D. Stenger;R. Kubiniec;S. Hui
中科院分区：
文献类型：
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作者：
H. Liang;W. J. Purucker;D. Stenger;R. Kubiniec;S. Hui
在电场脉冲作用下，粘附的10t1 /2小鼠成纤维细胞对荧光标记右旋糖酐的摄取被用作电渗透效率的标准。用同轴电极浸泡在培养皿中，对单层细胞进行渗透。在独立变化的脉冲场强和脉冲长度下，测量了电场脉冲应用后表现出荧光吸收的细胞百分比。分子量等于或大于41,000道尔顿的Dextrans需要更高的场强或更长的脉冲时间才能穿透细胞。使用矩形脉冲与使用类似功率的指数衰减脉冲相比，没有明显的优势。在40-950微秒和0.1-14.5 kV/cm的实验范围内，吸收与脉冲幅度和持续时间的乘积成正比。在这个范围的上端，细胞存活率下降。结果提供了一个直接比较的电参数，到目前为止还没有标准化的关于细胞电渗透。
The uptake of fluorescence-labeled dextrans by adherent 10T 1/2 murine fibroblasts following electric field pulse application was used as a criterion for the efficiency of electropermeation. The cells in monolayers were permeated by immersing a coaxial electrode in culture dishes. The percentage of cells which exhibited fluorescence uptake following electric field pulse application was measured at independently varying pulse field strength and pulse length. Dextrans with molecular weights equal to or higher than 41,000 dalton require higher field strength or longer pulse time to penetrate the cells. There is no detectable advantage of using a rectangular pulse against using an exponential decay pulse of similar power. The uptake was proportional to the product of the pulse amplitude and duration over the experimental range of 40-950 microseconds and 0.1-14.5 kV/cm. Cell survival decreases at the upper end of this range. The result provides a direct comparison of electric parameters which so far have not been standardized with regard to cell electropermeation.