Gene expression profiles of bovine trophoblastic cell line (BT-1) analyzed by a custom cDNA microarray.
Gene expression profiles of bovine trophoblastic cell line (BT-1) analyzed by a custom cDNA microarray.
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DOI：
10.1262/jrd.16072
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发表时间：
2005-04
期刊：
影响因子：
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通讯作者：
K. Ushizawa;Toru Takahashi;K. Kaneyama;T. Tokunaga;Y. Tsunoda;K. Hashizume
中科院分区：
文献类型：
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作者：
K. Ushizawa;Toru Takahashi;K. Kaneyama;T. Tokunaga;Y. Tsunoda;K. Hashizume
利用定制的子宫胎盘互补基因芯片分析了牛滋养层细胞系BT-1的基因表达。进行与滋养层衍生的体内组织的表达比较以研究BT-1中的表达特征。BT-1是使用从牛胚泡分离的滋养层细胞在没有饲养细胞的情况下建立的细胞系。在妊娠第17天至第56天收集滋养层来源的牛体内组织。在1，773个基因中，933个在BT-1和体内组织之间表现出超过两倍的表达差异。这些基因进行了分析，通过k-means聚类方法，并分布到六个集群。在整个实验期间，在BT-1以及体内组织中检测到一些基因，如胎盘催乳素、干扰素-tau和妊娠相关糖蛋白，它们都被称为滋养层特异性基因。这些滋养层特异性基因和八聚体结合转录因子-4，被称为小鼠细胞未分化的标志物，通过RT-PCR在BT-1和体内滋养层组织中检测到。BT-1中的整体基因表达谱表明，该细胞系含有滋养层特异性特征，并且与着床期周围的滋养层细胞相似。
Gene expression of bovine trophoblast cell line (BT-1) was analyzed with a custom utero-placenta complementary DNA microarray. Expression comparison with in vivo tissues of trophoblast derivation was performed to investigate characteristics of the expression in BT-1. BT-1 is a cell line established without feeder cells using trophoblast cells that are separated from bovine blastocysts. The bovine in vivo tissues of the trophoblast derivation were collected on day 17 through 56 of gestation. Of 1,773 genes, 933 exhibited an expression difference exceeding two-fold between BT-1 and in vivo tissues. These genes were analyzed by the k-means clustering method and were distributed into six clusters. Some genes, such as placental lactogen, interferon-tau and pregnancy-associated glycoproteins, all known as trophoblast-specific genes, were detected in BT-1 as well as in vivo tissues throughout the experiment period. These trophoblast-specific genes and octamer-binding transcription factor-4, known as a marker for undifferentiation of cells in mice, were detected by RT-PCR in both BT-1 and in vivo trophoblast tissues. The overall gene expression profile in BT-1 suggests that this cell line contains trophoblast-specific characteristics and is similar to trophoblast cells around the implantation period.