Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults
Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults
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DOI：
10.1111/j.1601-0825.2012.01944.x
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发表时间：
2012-11-01
期刊：
影响因子：
3.8
通讯作者：
Yamashita, Y.
中科院分区：
文献类型：
--
作者：
Li, H.;Takeshita, T.;Yamashita, Y.
Oral Diseases（2012）18，771777目的：描述虚弱老年人口腔真菌种群的全球组成，并研究其与健康状况的关系。材料与方法：我们调查了291名机构化老年人舌背真菌种群的分子PCR为基础的技术，使用核核糖体DNA的内部转录间隔区。结果如下：定量PCR分析表明，真菌存在于舌背上的128个受试者在= 104 CFU/样品，其中35个超过105 CFU/样品。长度异质性-PCR分析和核苷酸序列测定显示，白色念珠菌最常在真菌≥ 104 CFU/样本的受试者中检出（105例受试者），其次是都柏林念珠菌（78例）、限制性马拉色菌（57例）和热带念珠菌（45例）。统计学分析显示，与每个样本中含有≥ 105 CFU真菌（除白色念珠菌外）的受试者相比，每个样本中含有≥ 105 CFU真菌（除白色念珠菌外）的受试者的发热风险增加（≥ 7个发热日/12个月）。
Oral Diseases (2012) 18, 771777 Objectives: To characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status. Materials and methods: We investigated the fungal populations on the tongue dorsum in 291 institutionalized elderly adults by molecular PCR-based techniques using internal transcribed spacer regions of nuclear ribosomal DNA. Results: Quantitative PCR analysis showed that fungi were present on the tongue dorsum of 128 subjects at =104 CFU per sample, and 35 of them exceeded 105 CFU per sample. Length heterogeneity-PCR analysis and nucleotide sequence determinations showed that Candida albicans was most frequently detected in those subjects with fungi at =104 CFU per sample (105 subjects), followed by Candida dubliniensis (78), Malassezia restricta (57), and Candida tropicalis (45). Statistical analysis revealed that those subjects with =105 CFU of fungi other than C.similar to albicans per sample had an increased risk of fever (=7 febrile days per 12 months) compared with subjects with