Inability of Escherichia coli to resuscitate from the viable but nonculturable state
Inability of Escherichia coli to resuscitate from the viable but nonculturable state
复制标题
DOI：
10.1111/j.1574-6941.2007.00362.x
复制
发表时间：
2007-10-01
影响因子：
4.2
通讯作者：
Barcina, Isabel
中科院分区：
文献类型：
--
作者：
Arana, Ines;Orruno, Maite;Barcina, Isabel
在诱导大肠杆菌种群的活的但不可培养（VBNC）状态后，我们分析了促进复苏过程的非生物和生物因素。对胁迫条件的反应意味着三个亚群的形成，可培养的，VBNC和非可行的。在研究的大多数不良情况下，VBNC亚群并不代表占主导地位的部分，随着时间的推移而减少。这表明，在大多数情况下，VBNC不是一个成功的表型。结合稀释和抑制剩余可培养细胞的方法，我们设计了一个工作方案，以明确区分再生和复苏。逆转非生物因素诱导nonculturability以及预防额外的氧化应激没有引起复苏。通过添加来自不同来源的上清液研究生物因子的参与，但未获得阳性结果。这些结果表明，E.所用的大肠杆菌菌株不能从VBNC状态复苏。VBNC细胞释放到周围培养基中，因此可以帮助持续培养的细胞存活。因此，VBNC亚群的形成可以被视为一个适应过程，旨在为整个人口的利益。
After induction of the viable but nonculturable (VBNC) state in Escherichia coli populations, we analysed abiotic and biotic factors suggested to promote the resuscitation process. The response to the stressing conditions implied the formation of three subpopulations, culturable, VBNC and nonviable. In most adverse situations studied, the VBNC subpopulation did not represent the dominant fraction, decreasing with time. This suggests that, in most cases, the VBNC is not a successful phenotype. Combining methods of dilution and inhibition of remaining culturable cells, we designed a working protocol in order to distinguish unequivocally between regrowth and resuscitation. Reversion of abiotic factors inducing nonculturability as well as prevention of additional oxidative stress did not provoke resuscitation. Participation of biotic factors was studied by addition of supernatants from different origin without positive results. These results indicate that the E. coli strain used is not able to resuscitate from the VBNC state. VBNC cells release into the surrounding medium, and could thus aid in the survival of persisting culturable cells. The formation of a VBNC subpopulation could thus be considered as an adaptive process, designed for the benefit of the population as a whole.