Characterization of monoclonal antibodies to human melanoma-associated antigens.
Characterization of monoclonal antibodies to human melanoma-associated antigens.
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人类黑色素瘤相关抗原单克隆抗体的表征。
DOI：
10.1097/00006534-198100000-00138
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
S. Ferrone
中科院分区：
文献类型：
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作者：
K. Imai;A. Ng;S. Ferrone
来自骨髓瘤细胞与来自用培养的人黑素瘤细胞免疫的小鼠的脾细胞的融合的杂交瘤No. 165.28 T、No. 473.54 S和No. 653.25 N分泌识别在培养的人黑素瘤细胞和新鲜分离的黑素瘤细胞上最大表达的抗原决定簇的单克隆抗体。单克隆抗体376.74T也与癌细胞系反应，但滴度显著较低。玫瑰花结抑制试验表明，这些抗原决定簇表达在抗原结构上，与β 2微球蛋白和组织相容性抗原无关。两种单克隆抗体识别相同或密切相关的抗原决定簇，其余两种单克隆抗体与不同的抗原决定簇反应。所有四种单克隆抗体都可以介导培养的黑色素瘤细胞的抗体依赖性细胞毒性，但没有一种可以介导补体依赖性细胞毒性。
The hybridomas No. 165.28T, No. 473.54S, and No. 653.25N derived from the fusion of myeloma cells with splenocytes from mice immunized with cultured human melanoma cells secreted monoclonal antibodies recognizing antigenic determinants maximally expressed on cultured human melanoma cells and freshly explanted melanoma cells. Monoclonal antibody No. 376.74T reacted also with carcinoma cell lines but with a significantly lower titer. Rosette inhibition assay showed that these antigenic determinants were expressed on antigenic structures, which are not associated with beta 2 microglobulin and histocompatibility antigens. Two monoclonal antibodies recognized the same or closely associated antigenic determinants, and the remaining two monoclonal antibodies reacted with distinct antigenic determinants. All four monoclonal antibodies could mediate antibody-dependent cellular cytotoxicity of cultured melanoma cells, but none could mediate complement-dependent cytotoxicity.