OLFML3 expression is decreased during prenatal muscle development and regulated by microRNA-155 in pigs.
OLFML3 expression is decreased during prenatal muscle development and regulated by microRNA-155 in pigs.
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OLFML3 表达在猪产前肌肉发育过程中下降并受 MicroRNA-155 调节
DOI：
10.7150/ijbs.3821
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发表时间：
2012
影响因子：
9.2
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Zhao S;Zhang J;Hou X;Zan L;Wang N;Tang Z;Li K
Olfactomedin-like 3（OLFML 3）基因具有参与胚胎发育的基质相关功能。MicroRNA-155（miR-155）是一种21- 23个核苷酸的非编码RNA，通过靶mRNA调控肌细胞的发生。LongSAGE分析结果表明，OLFML 3基因在猪肌肉发育过程中存在差异表达。本研究克隆了猪OLFML 3基因，并检测了该基因在成年通城猪中的组织分布以及在长白猪（瘦肉型）和通城猪（肥胖型）骨骼肌发育（12个产前和10个产后阶段）中的动态表达。随后，我们分析了OLFML 3和miR-155之间的相互作用。OLFML 3在肝脏和胰腺中表达丰富，在肺、小肠和胎盘中表达中等，在其他组织和生后肌肉中表达较弱。长白猪和通城猪骨骼肌在产前发育过程中存在不同的动态表达模式。OLFML 3在长白猪中下调（交配后33-50天，dpc），随后上调（50-70 dpc），然后下调（70-100 dpc），而在通城猪中下调（33-50 dpc），随后上调（50-55 dpc），然后下调（55-100 dpc）。在33 ~ 60 dpc，桐城猪的表达量高于长白猪，而在65 ~ 100 dpc，桐城猪的表达量高于长白猪。双荧光素酶法和真实的时间PCR证实OLFML 3的表达受miR-155在mRNA水平的调控。我们的研究表明OLFML 3基因可能影响胎儿骨骼肌的发育，并受miR-155的调控。这些发现将有助于了解OLFML 3基因在哺乳动物中的生物学功能和表达调控。
The Olfactomedin-like 3 (OLFML3) gene has matrix-related function involved in embryonic development. MicroRNA-155 (miR-155), 21- to 23-nucleotides (nt) noncoding RNA, regulated myogenesis by target mRNA. Our LongSAGE analysis suggested that OLFML3 gene was differently expressed during muscle development in pig. In this study, we cloned the porcine OLFML3 gene and detected its tissues distribution in adult Tongcheng pigs and dynamical expression in developmental skeletal muscle (12 prenatal and 10 postnatal stages) from Landrace (lean-type) and Tongcheng (obese-type) pigs. Subsequently, we analyzed the interaction between OLFML3 and miR-155. The OLFML3 was abundantly expressed in liver and pancreas, moderately in lung, small intestine and placenta, and weakly in other tissues and postnatal muscle. There were different dynamical expression patterns between Landrace and Tongcheng pigs during prenatal skeletal muscle development. The OLFML3 was down-regulated (33-50 days post coitus, dpc), subsequently up-regulated (50-70 dpc), and then down-regulated (70-100 dpc) in Landrace pigs, while in Tongcheng pigs, it was down-regulated (33-50 dpc), subsequently up-regulated (50-55 dpc) and then down-regulated (55-100 dpc). There was higher expression in Tongcheng than Landrace in prenatal muscle from 33 to 60 dpc, and opposite situation from 65 to 100 dpc. Dual luciferase assay and real time PCR documented that OLFML3 expression was regulated by miR-155 at mRNA level. Our research indicated that OLFML3 gene may affect prenatal skeletal muscle development and was regulated by miR-155. These finding will help understanding biological function and expression regulation of OLFML3 gene in mammal animals.
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DOI：
10.1083/jcb.200603008
发表时间：
2006-08-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kim HK;Lee YS;Sivaprasad U;Malhotra A;Dutta A
通讯作者：
Dutta A
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
DOI：
10.1083/jcb.201007165
发表时间：
2011-01-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ge Y;Sun Y;Chen J
通讯作者：
Chen J
影响因子：
3.4
作者：
Paper, Walter;Kroeber, Markus;Tamm, Ernst R.
通讯作者：
Tamm, Ernst R.
DOI：
10.1083/jcb.200210053
发表时间：
2003-02-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hillier BJ;Vacquier VD
通讯作者：
Vacquier VD