Subcellular localization of the Schlafen protein family
Subcellular localization of the Schlafen protein family
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DOI：
10.1016/j.bbrc.2008.03.032
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发表时间：
2008-05-23
影响因子：
3.1
通讯作者：
Gonda, Thomas J.
中科院分区：
文献类型：
--
作者：
Neumann, Brent;Zhao, Liang;Gonda, Thomas J.
虽然Schlafen基因家族的第一个成员在近10年前被首次描述，但它们编码的蛋白质的精确分子/生化功能在很大程度上仍然未知。在细胞生长、造血细胞分化和T细胞发育/成熟中的作用，有一些实验支持，但没有一个得到最终证实。在这里，我们确定了Schlafens 1、2、4、5、8和9的亚细胞定位，代表了所有三个小鼠亚群。我们发现来自亚群I和II的蛋白质定位于细胞质，而亚群III的较长形式仅定位于核室。我们还证实了Schlafen2在造血细胞分化过程中的上调，并表明这种内源性蛋白定位于细胞质。因此，我们提出Schlafen蛋白的不同亚群可能具有fu (c) 2008 Elsevier Inc.。版权所有。
Although the first members of the Schlafen gene family were first described almost 10 years ago, the precise molecular/biochemical functions of the proteins they encode still remain largely unknown. Roles in cell growth, haematopoietic cell differentiation, and T cell development/maturation have, with some experimental support, been postulated, but none have been conclusively verified. Here, we have determined the subcellular localization of Schlafens 1, 2, 4, 5, 8, and 9, representing all three of the murine subgroups. We show that the proteins from subgroups I and II localize to the cytoplasm, while the longer forms in subgroup III localize exclusively to the nuclear compartment. We also demonstrate upregulation of Schlafen2 upon differentiation of haematopoietic cells and show this endogenous protein localizes to the cytoplasm. Thus, we propose the different subgroups of Schlafen proteins are likely to have fu (c) 2008 Elsevier Inc. All rights reserved.