Detection of enterovirus in the thyroid tissue of patients with graves' disease
Detection of enterovirus in the thyroid tissue of patients with graves' disease
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DOI：
10.1002/jmv.23476
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发表时间：
2013-03-01
影响因子：
12.7
通讯作者：
Dahl-Jorgensen, Knut
中科院分区：
文献类型：
--
作者：
Hammerstad, Sara Salehi;Tauriainen, Sisko;Dahl-Jorgensen, Knut
Graves病（GD）的病因和发病机制仍然是未知的，虽然它被认为是遗传和环境因素都很重要。一些间接证据表明，病毒感染可能是自身免疫的一个可能的病因。本研究的主要目的是检查GD患者甲状腺组织中存在肠道病毒（EV）的直接证据。通过粗针穿刺活检获得22例新诊断GD患者的甲状腺组织，同时在颈部手术期间收集24例慢性GD患者和24例无任何自身免疫性甲状腺疾病的对照组的组织。福尔马林固定，石蜡包埋的甲状腺组织样本进行了检查肠道病毒衣壳蛋白的存在下，使用免疫组化和肠道病毒RNA使用原位杂交。17例（37%）患者和4例（17%）对照受试者中检测到肠道病毒衣壳蛋白（P = 0.001）。0.103）。在9例（20%）患者的甲状腺组织中发现了肠道病毒RNA，但在对照组中没有发现肠道病毒RNA（P = 0.001）。0.016）。9例病毒RNA阳性患者中有8例（90%）肠道病毒蛋白阳性。这是第一项分析甲状腺组织中EV的研究，包括未经治疗的新诊断GD患者。结果表明，EV在患者甲状腺组织中的存在频率比对照组更高。进一步的研究表明，探索这种关联，以找出一个低级别的慢性肠道病毒感染可能参与GD的发病机制，如果这可以提供新的治疗和预防的机会。J. Med. Virol. 85：512518，2013。(c)2012 Wiley Periodicals，Inc.
The etiology and pathogenesis of Graves' disease (GD) are still unknown, although it is thought that both genetic and environmental factors are important. Some indirect evidence implies that a viral infection may be a possible etiologic factor in autoimmunity. The main objective of this study was to examine direct evidence of the presence of enteroviruses (EVs) in the thyroid tissue of patients with GD. Thyroid tissue from 22 patients with newly diagnosed GD was obtained by core needle biopsy, while tissue from 24 patients with chronic GD and 24 control subjects without any autoimmune thyroid diseases was collected during neck surgery. Formalin-fixed, paraffin-embedded thyroid tissue samples were examined for the presence of enterovirus capsid protein using immunohistochemistry and for enterovirus RNA using in situ hybridization. Enterovirus capsid protein was detected in 17 (37%) patients and in 4 (17%) control subjects (P?=?0.103). Enterovirus RNA was identified in thyroid tissue from nine (20%) patients, but in none of the control subjects (P?=?0.016). Eight (90%) of the nine virus RNA positive patients were also positive for enterovirus protein. This is the first study to analyze thyroid tissue for EVs, including patients with untreated, newly diagnosed GD. The results suggest that EVs are more frequently present in thyroid tissue of patients than controls. Further studies are indicated to explore this association to find out if a low-grade chronic enteroviral infection might be involved in the pathogenesis of GD and if this could offer new therapeutic and preventive opportunities. J. Med. Virol. 85:512518, 2013. (c) 2012 Wiley Periodicals, Inc.