Non-Photochemical Quenching Capacity in Arabidopsis thaliana Affects Herbivore Behaviour
Non-Photochemical Quenching Capacity in Arabidopsis thaliana Affects Herbivore Behaviour
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DOI：
10.1371/journal.pone.0053232
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发表时间：
2013-01-02
期刊：
影响因子：
3.7
通讯作者：
Jansson, Stefan
中科院分区：
文献类型：
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作者：
Jankanpaa, Hanna Johansson;Frenkel, Martin;Jansson, Stefan
在自然条件下，植物必须科普许多压力，包括光胁迫和植食性。这就提出了关于压力防御和增长之间可能的权衡的有趣问题。作为旨在解决这些问题的程序的一部分，我们比较了野生型拟南芥和两个“光保护基因型”，npq 4和oePsbS，分别缺乏和过表达PsbS（一种蛋白质，在QE型非光化学猝灭中起着关键作用）的草食性防御和损害。在双重选择喂养实验中，专家（小菜蛾）和通才（Spodoptera littoralis）昆虫食草动物的首选植物，表达PSBS最强烈。相反，虽然这两种草食动物同样存活良好的基因型，产卵雌性小菜蛾成虫更喜欢的植物，表达PsbS最不强烈。然而，有10个最突出的硫代葡萄糖苷的水平基因型之间没有显着差异，关键物质在拟南芥抗草食动物的化学防御武器库。在从生长室转移到田间后，我们使用GC-MS检测到在所有测试时间点基因型代谢组学谱的显著差异，但对于缺乏PsbS没有一致的“代谢特征”。这些发现表明，所观察到的草食动物偏好的差异是由于在植物的初级代谢的差异，而不是其内容的典型的“防御化合物”。一个潜在的重要因素是npq 4突变体在强光条件下超氧化物积累最快，达到最高水平。这可能会引发植物的变化，这些变化被食草动物直接或间接地感知，随后其歧化为H2 O2。
Under natural conditions, plants have to cope with numerous stresses, including light-stress and herbivory. This raises intriguing questions regarding possible trade-offs between stress defences and growth. As part of a program designed to address these questions we have compared herbivory defences and damage in wild type Arabidopsis thaliana and two "photoprotection genotypes", npq4 and oePsbS, which respectively lack and overexpress PsbS (a protein that plays a key role in qE-type non-photochemical quenching). In dual-choice feeding experiments both a specialist (Plutella xylostella) and a generalist (Spodoptera littoralis) insect herbivore preferred plants that expressed PsbS most strongly. In contrast, although both herbivores survived equally well on each of the genotypes, for oviposition female P. xylostella adults preferred plants that expressed PsbS least strongly. However, there were no significant differences between the genotypes in levels of the 10 most prominent glucosinolates; key substances in the Arabidopsis anti-herbivore chemical defence arsenal. After transfer from a growth chamber to the field we detected significant differences in the genotypes' metabolomic profiles at all tested time points, using GC-MS, but no consistent "metabolic signature'' for the lack of PsbS. These findings suggest that the observed differences in herbivore preferences were due to differences in the primary metabolism of the plants rather than their contents of typical "defence compounds". A potentially significant factor is that superoxide accumulated most rapidly and to the highest levels under high light conditions in npq4 mutants. This could trigger changes in planta that are sensed by herbivores either directly or indirectly, following its dismutation to H2O2.