Prehydration mitigates damage accrued from prolonged periods of desiccation in cultured shoot apices of Syntrichia ruralis
Prehydration mitigates damage accrued from prolonged periods of desiccation in cultured shoot apices of Syntrichia ruralis
复制标题
DOI：
10.1080/03736687.2020.1833157
复制
发表时间：
2020-12
影响因子：
1.9
通讯作者：
Mandy L. Slate;J. Brinda;K. Coe;Joshua Greenwood;L. Stark
中科院分区：
文献类型：
--
作者：
Mandy L. Slate;J. Brinda;K. Coe;Joshua Greenwood;L. Stark
摘要介绍。我们假设，预水化期（暴露在潮湿的空气中）应该减轻沙漠苔藓（Syntrichia rural alis）短时间或长时间干燥的破坏性影响。方法。将培养的农家草单克隆芽快速干燥，在42%的相对湿度（RH）下保持1 ~ 50天，然后(1)在100% RH下预水化24 h，然后用液态水再水化，或(2)直接用液态水再水化，不进行预水化处理，用叶绿素荧光和视觉损伤来评估。关键的结果。在水化后20 min和24 h，预水化苗的所有叶绿素荧光测量值（F m、F v/F m、ΦPSII、qP）均较高。水化处理后7 d，预水化处理后的农村野刺幼苗叶片视觉损伤也较小。干燥持续时间（DD）对补液后立即对芽的所有荧光测量都有总体负面影响，在补液后24小时，四种荧光测量中的三种（F m, F v/F m， ΦPSII）的影响消失。叶片损伤不受干燥时间的影响。虽然没有发现预水化和持续干燥处理之间的相互作用，但根据荧光指标F v/F m和ΦPSII，当芽暴露于较长的干燥时间时，预水化的好处得到了强调。结论。在与液态水水化之前进行预水化处理，可以显著减轻先前快速干燥事件造成的茎部损伤，并且在42%相对湿度下，dd可达50 d。基于光合效率，预水合作用在更长的DDs上具有更大的效果。在雨水之前经历潮湿的环境可以减轻长时间干燥造成的损害。
ABSTRACT Introduction. We hypothesised that a prehydration period (exposure to humid air) should mitigate the damaging effects of short or long periods of desiccation in a desert moss, Syntrichia ruralis. Methods. Cultured uniclonal shoots of S. ruralis were dried rapidly, equilibrated at 42% relative humidity (RH), allowed to remain at this RH for 1–50 days, and either (1) prehydrated (24 h at 100% RH) then rehydrated with liquid water, or (2) rehydrated directly using liquid water without a prehydration treatment, and assessed using chlorophyll fluorescence and visual damage. Key results. At both 20 min and 24 h postrehydration, all chlorophyll fluorescence measures (F m, F v/F m, ΦPSII, qP) were higher in shoots that were prehydrated. Prehydrated S. ruralis shoots also had less visual leaf damage 7 d postrehydration. Duration-dry (DD) had an overall negative effect for all fluorescence measures on shoots immediately after rehydration, an effect that dissipated at 24 h postrehydration in three of the four fluorescence measures (F m, F v/F m, ΦPSII). Leaf damage was not influenced by Duration-dry. Although no interaction was detected between Prehydration and Duration-dry treatments, the benefits of prehydration were accentuated based on fluorescence metrics F v/F m and ΦPSII, when shoots are exposed to longer drying periods. Conclusions. A prehydration treatment implemented just prior to hydration with liquid water significantly mitigated shoot damage accrued from a prior rapid drying event and DDs of up to 50 d at 42% RH. Based on photosynthetic efficiency, prehydration conferred greater effects at longer DDs. Experiencing humid conditions prior to rainwater mitigates damage incurred during prolonged desiccation.