Cranial bone regeneration using a composite scaffold of beta-tricalcium phosphate, collagen, and autologous bone fragments
Cranial bone regeneration using a composite scaffold of beta-tricalcium phosphate, collagen, and autologous bone fragments
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DOI：
10.1097/01.mlg.0000191468.45536.3f
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发表时间：
2006-02-01
期刊：
影响因子：
2.6
通讯作者：
Ito, J
中科院分区：
文献类型：
--
作者：
Kishimoto, M;Kanemaru, S;Ito, J
Objective.本研究的目的是检查植入颅骨缺损的β-磷酸三钙（β-TCP）、胶原蛋白和自体骨碎片与纤维蛋白胶诱导的组织反应和新骨形成。材料和方法。将来自猪皮的颗粒状β-TCP和胶原蛋白混合并冷冻干燥。两种材料的重量比为98：2。将该人工材料与自体骨碎片复合制成骨再生复合支架。使用了5只成年比格犬。在每只犬中创建颅骨缺损（2 cm x 2 cm）。将动物分为两组。第1组（n = 3）用原游离骨瓣替代颅骨缺损，用含纤维蛋白胶的复合支架材料填充残余裂隙和钻孔。第2组（n = 2）仅通过替换原游离骨瓣来闭合骨缺损。治疗后3个月，对所有动物进行计算机断层扫描和组织学检查。结果如下：在第1组中，所有犬的缺损几乎被新骨封闭;而在第2组中，缺损被纤维组织而不是骨填充。结论：该研究表明，由β-TCP、胶原蛋白和自体骨碎片与纤维蛋白胶制成的复合支架能够在不使用常用固定材料的情况下重建颅骨缺损。
Objective. The aim of our study was to examine the tissue response and new bone formation induced by beta-tricalcium phosphate (beta-TCP), collagen, and autologous bone fragments with fibrin glue implanted into a cranial bone defect. Materials and Methods. Granulated beta-TCP and collagen from porcine skin were mixed and freeze-dried. The weight ratio of both materials was 98:2. The composite scaffold for bone regeneration was composed of this artificial material and autologous bone fragments. Five adult beagle dogs were used. A cranial bone defect (2 cm x 2 cm) was created in each dog. The animals were divided into two groups. In group 1 (n = 3), the cranial bone defect was closed by replacing the original free bone flap, and the residual fissure and burr holes were filled with the composite scaffold with fibrin glue. In group 2 (n = 2), the bone defect was closed only by replacing the original free bone flap. Three months after the treatment, computed tomography and histologic examinations were done in all animals. Results: In all dogs in group 1, the defects were almost closed by new bone; whereas in group 2, the defects were filled with fibrous tissues instead of bone. Conclusion: This study showed that the composite scaffold made of beta-TCP, collagen, and autologous bone fragments with fibrin glue enabled reconstruction of cranial bone defects without the usual fixation materials.