ACTIVITY OF KREBS CYCLE ENZYMES IN mdx MICE
ACTIVITY OF KREBS CYCLE ENZYMES IN mdx MICE
复制标题
DOI：
10.1002/mus.24704
复制
发表时间：
2016-01-01
期刊：
影响因子：
3.4
通讯作者：
Quevedo, Joao
中科院分区：
文献类型：
--
作者：
Comim, Clarissa M.;Hoepers, Andreza;Quevedo, Joao
简介：杜氏肌营养不良症（DMD）是一种由肌营养不良蛋白基因缺陷引起的骨骼肌、呼吸肌和心肌退行性疾病。最近，大脑参与已经得到证实。线粒体功能障碍和氧化应激可能是DMD的病理生理基础。在这项研究中，我们评估克雷布斯循环酶活性的大脑皮层，横膈膜，和四头肌的mdx小鼠。方法：使用来自雄性营养不良mdx和对照小鼠的皮质、隔膜和四头肌组织。结果如下：我们观察到增加苹果酸脱氢酶活性在皮质;增加苹果酸脱氢酶和琥珀酸脱氢酶活性在横膈膜;和增加柠檬酸合成酶，异柠檬酸脱氢酶，苹果酸脱氢酶活性在mdx小鼠的四头肌。结论：本研究显示mdx小鼠皮质、四头肌和膈肌中Krebs循环酶的活性增加。
Introduction: Duchenne muscular dystrophy (DMD) is a degenerative disease of skeletal, respiratory, and cardiac muscles caused by defects in the dystrophin gene. More recently, brain involvement has been verified. Mitochondrial dysfunction and oxidative stress may underlie the pathophysiology of DMD. In this study we evaluate Krebs cycle enzymes activity in the cerebral cortex, diaphragm, and quadriceps muscles of mdx mice. Methods: Cortex, diaphragm, and quadriceps tissues from male dystrophic mdx and control mice were used. Results: We observed increased malate dehydrogenase activity in the cortex; increased malate dehydrogenase and succinate dehydrogenase activities in the diaphragm; and increased citrate synthase, isocitrate dehydrogenase, and malate dehydrogenase activities in the quadriceps of mdx mice. Conclusion: This study showed increased activity of Krebs cycle enzymes in cortex, quadriceps, and diaphragm in mdx mice.