Involvement of Apelin/APJ Axis in Thrombogenesis in Valve Heart Disease Patients with Atrial Fibrillation
Involvement of Apelin/APJ Axis in Thrombogenesis in Valve Heart Disease Patients with Atrial Fibrillation
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DOI：
10.1536/ihj.18-166
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发表时间：
2019-01-01
影响因子：
1.5
通讯作者：
Xu, Biao
中科院分区：
文献类型：
--
作者：
Cheng, Hai;Chen, Yuhan;Xu, Biao
心房颤动(房颤)中血栓形成的机制尚不清楚。Apelin/APJ轴与血管紧张素系统平行和反向调节。本研究假设APELIN/APJ轴在正常左心房组织中发挥抗血栓作用，并在房颤时被上调的肾素血管紧张素-醛固酮系统(RAAS)信号干扰。收集风湿性二尖瓣狭窄瓣膜置换术患者的左心耳标本，分为窦性心律、房颤(+)/血栓(-)、房颤(+)/血栓(+)三组。免疫印迹法检测血管紧张素II受体亚型1(AT1)、apelin/APJ及其下游纤溶酶原激活物抑制物-1(PAI-1)的含量。房颤(+)/血栓(+)组apelin/APJ的表达明显低于窦性心律和房颤(+)/血栓(-)组。同时，房颤(+)/血栓(+)组AT1和PAI-1的表达高于其他两组。综上所述，本研究揭示APELIN/APJ轴可能与PM-1介导的房颤患者血栓形成有关。
The mechanism underlying thrombosis in atrial fibrillation (AF) is not yet clearly understood. The apelin/APJ axis parallel and counter-regulate with the angiotensin system. The present study hypothesizes that apelin/APJ axis exert its anti-thrombus effect in normal left atrial tissue and is disrupted by up-regulated reninangiotensin-aldosterone system (RAAS) signaling during AF. The specimens of left atrial appendages collected from patients with rheumatic mitral stenosis who underwent valve replacement were divided into 3 groups: sinus rhythm, AF(+)/thrombus(-), and AF(+)/thrombus(+). The amounts of angiotensin II receptor subtype 1 (AT1), apelin/APJ and its downstream plasminogen activator inhibitor-1 (PAI-1) were detected by western blot. The expression of apelin/APJ was significantly decreased in the AF(+)/thrombus(+) group compared with the sinus rhythm and AF(+)/thrombus(-) groups. Meanwhile the expressions of AT1 and PAI-1 were highest in the AF(+)/thrombus(+) group compared to the other two groups. Taken together, the present study reveals apelin/APJ axis might be correlated with thrombosis in patients with AF mediated by PM-1.