Physioxia Stimulates Extracellular Matrix Deposition and Increases Mechanical Properties of Human Chondrocyte-Derived Tissue-Engineered Cartilage.
Physioxia Stimulates Extracellular Matrix Deposition and Increases Mechanical Properties of Human Chondrocyte-Derived Tissue-Engineered Cartilage.
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DOI：
10.3389/fbioe.2020.590743
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发表时间：
2020
影响因子：
5.7
通讯作者：
Kean TJ
中科院分区：
文献类型：
--
作者：
Dennis JE;Whitney GA;Rai J;Fernandes RJ;Kean TJ
软骨组织一直是组织工程方法的顽固性。在这项研究中，将人软骨细胞形成自组装软骨片，在生理性（5%）和常压（20%）氧气条件下培养，并在1个月和2个月时进行生化、组织学和生物力学分析。结果表明，与常压培养相比，生理氧张力下形成的薄片更厚，含有更多的糖胺聚糖（GAGs）和II型胶原蛋白，具有更好的压缩和拉伸性能。在所有病例中，软骨片染色，细胞外基质成分。在新生软骨和原纤维中形成的II-IX-XI型胶原异质聚合物被三价吡啶啉交联稳定。胶原交联不受氧张力的显著影响，但随着培养时间的延长而增加。生理氧张力和较长的培养时间都有助于增加细胞外基质成分。最重要的相关性是发现压缩刚度和GAG与胶原蛋白的比例。
Cartilage tissue has been recalcitrant to tissue engineering approaches. In this study, human chondrocytes were formed into self-assembled cartilage sheets, cultured in physiologic (5%) and atmospheric (20%) oxygen conditions and underwent biochemical, histological and biomechanical analysis at 1- and 2-months. The results indicated that sheets formed at physiological oxygen tension were thicker, contained greater amounts of glycosaminoglycans (GAGs) and type II collagen, and had greater compressive and tensile properties than those cultured in atmospheric oxygen. In all cases, cartilage sheets stained throughout for extracellular matrix components. Type II-IX-XI collagen heteropolymer formed in the neo-cartilage and fibrils were stabilized by trivalent pyridinoline cross-links. Collagen cross-links were not significantly affected by oxygen tension but increased with time in culture. Physiological oxygen tension and longer culture periods both served to increase extracellular matrix components. The foremost correlation was found between compressive stiffness and the GAG to collagen ratio.
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影响因子：
6
作者：
Kean, Thomas J.;Ge, Zhongqi;Dennis, James E.
通讯作者：
Dennis, James E.
影响因子：
3.7
作者：
Kean, Thomas J.;Dennis, James E.
通讯作者：
Dennis, James E.
影响因子：
--
作者：
Enochson L;Brittberg M;Lindahl A
通讯作者：
Lindahl A
影响因子：
2.8
作者：
Markway, Brandon D.;Cho, Holly;Johnstone, Brian
通讯作者：
Johnstone, Brian
影响因子：
4.1
作者：
Huang, Brian J.;Brown, Wendy E.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.