Membrane crystals of plant light-harvesting complex II disassemble reversibly in light.
Membrane crystals of plant light-harvesting complex II disassemble reversibly in light.
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DOI：
10.1093/pcp/pcu064
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发表时间：
2014-07
影响因子：
4.9
通讯作者：
G. Hind;J. Wall;Z. Várkonyi;A. Istokovics;P. Lambrev;G. Garab
中科院分区：
文献类型：
--
作者：
G. Hind;J. Wall;Z. Várkonyi;A. Istokovics;P. Lambrev;G. Garab
利用扫描透射电子显微镜的质量测量能力，我们证明了植物的主要捕光复合物的膜晶体具有进行光诱导的暗可逆解离的能力，独立于光化学装置。这是第一个直接可视化的光驱动可逆重组在一个孤立的光合天线。这些重组，确定较早的圆二色性（CD），可以占由生物热光转换：结构变化引起的快速热瞬变和热不稳定性附近的耗散，和自缔合的复合物在脂质基质。在天然膜中的一个可比较的过程是由早期发现的基本相同的动力学，以及在基粒类囊体中的Δ CD的强度和温度依赖性所指示的。
Using the mass-measuring capability of scanning transmission electron microscopy, we demonstrate that membrane crystals of the main light-harvesting complex of plants possess the ability to undergo light-induced dark-reversible disassociations, independently of the photochemical apparatus. This is the first direct visualization of light-driven reversible reorganizations in an isolated photosynthetic antenna. These reorganizations, identified earlier by circular dichroism (CD), can be accounted for by a biological thermo-optic transition: structural changes are induced by fast heat transients and thermal instabilities near the dissipation, and self-association of the complexes in the lipid matrix. A comparable process in native membranes is indicated by earlier findings of essentially identical kinetics, and intensity and temperature dependences of the ΔCD in granal thylakoids.