Antibodies reactive to Trypanosoma cruzi epimastigotes or amastigotes express different idiotypic patterns if from patients with different clinical forms of Chagas' disease.
Antibodies reactive to Trypanosoma cruzi epimastigotes or amastigotes express different idiotypic patterns if from patients with different clinical forms of Chagas' disease.
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如果来自患有不同临床形式的恰加斯病的患者，对克氏锥虫上鞭毛体或无鞭毛体具有反应性的抗体表达不同的独特型模式。
DOI：
10.1046/j.1365-3083.1996.d01-262.x
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发表时间：
1996
影响因子：
3.7
通讯作者：
Carter,CE
中科院分区：
文献类型：
--
作者：
Ribeiro-Rodrigues,R;Colley,DG;Correa-Oliveira,R;Carter,CE
在表鞭毛体（EPI 或 E）或富含无鞭毛体（AMAST 或 A）抗原 (Ag) 柱上从不确定（IND 或 I）或心脏病（CARD 或 C）恰加斯病患者的混合血清中纯化抗体 (Ab)，并检查其独特型 (Id) 表达。针对不同的制剂（E-IdI、E-IdC、A-IdI 和 A-IdC）产生抗 Id 兔抗体。使用抗 Id 的竞争性 ELISA 能够区分 IdI 和 IdC，而无需考虑 Ag 反应性。 E-IdI和A-IdI表现出不同的抑制能力，E-IdC和A-IdC也表现出不同的抑制能力，但IdC总是与IdI竞争抗IdI。相比之下，IdI 需要增加 4-8 倍才能与 IdC 并行竞争抗 IdC。因此，来自 IND 患者的 Id 仅与 CARD 患者最有特征的 Id 具有较低水平。虽然一些 CARD Ab 也表达与 IND 患者相同的 Id，但这些研究表明 CARD Ab 表达一些仅 CARD 患者特有的 Id，并且这些 Id 存在于用 EPI 或 AMAST 纯化的 Ab 上。
Antibodies (Abs) were purified from pooled sera of patients with either indeterminate (IND or I) or cardiac (CARD or C) Chagas' disease, on either epimastigote (EPI or E) or amastigote‐enriched (AMAST or A) antigen (Ag) columns and their idiotypic (Id) expression examined. Anti‐Id rabbit Abs were raised to the different preparations (E‐IdI, E‐IdC, A‐IdI and A‐IdC). Competitive ELISAs using anti‐Ids were able to discriminate between IdI and IdC, disregarding Ag reactivity. E‐IdI and A‐IdI present different inhibitory abilities, as do E‐IdC and A‐IdC, but IdC always competes with IdI for anti‐IdI comparably. In contrast, a 4–8‐fold increase of IdI is required to compete in parallel with IdC for anti‐IdC. Therefore, Ids from IND patients share only low levels of the Ids that are most characteristic of CARD patients. While some CARD Abs also express Ids in common with IND patients, these studies reveal that CARD Abs express some Ids that are characteristic to only CARD patients, and these Ids are present on Abs purified with either EPI or AMAST.