Prolactin gene expression in human growth hormone-secreting pituitary adenomas.
Prolactin gene expression in human growth hormone-secreting pituitary adenomas.
复制标题
催乳素基因在分泌生长激素的垂体腺瘤中的表达。
作者：
T. Nagaya;H. Seo;A. Kuwayama;T. Sakurai;N. Tsukamoto;K. Sugita;N. Matsui
为探讨生长激素(GH)腺瘤患者常见的高催乳素血症的发病机制，采用免疫组织化学和细胞质斑点杂交技术检测了肿瘤组织中催乳素(ir-PRL)和催乳素(PRL)信使核糖核酸(MRNA)的表达。18例生长激素腺瘤中有3例出现高催乳素血症。9例肿瘤组织中含有ir-PRL，13例肿瘤组织中含有PRL mRNA。肿瘤组织中ir-PRL的存在总是与PRL mRNA阳性有关，提示在GH分泌型肿瘤中存在PRL。在3例高催乳素血症患者中，1例肿瘤组织中同时检测到ir-PRL和PRL mRNA，另1例腺瘤组织中仅检测到PRL mRNA而未检测到ir-PRL，第3例肿瘤组织中未检测到PRL和ir-PRL。高催乳素血症与ir-PRL和PRL信使核糖核酸的同时存在或仅与PRL信使核糖核酸的存在有关，提示PRL的产生和分泌。然而，肿瘤组织中ir-PRL和PRL mRNA的缺失可能表明高催乳素血症是由于肿瘤肿块对下丘脑功能障碍对PRL抑制因子的抑制所致。因此，研究催乳素基因在生长激素分泌腺瘤中的表达和免疫组织化学对了解垂体瘤的病理生理学有重要意义。
To elucidate the mechanism of hyperprolactinemia often observed in patients with growth hormone (GH)-secreting pituitary adenomas, the presence of immunoreactive prolactin (ir-PRL) and prolactin (PRL) messenger ribonucleic acid (mRNA) in the tumor tissue was examined by immunohistochemistry and cytoplasmic dot hybridization. Hyperprolactinemia was observed in three of 18 patients with GH-secreting adenoma. The tumor tissue was demonstrated to contain ir-PRL in nine patients and PRL mRNA in 13. The presence of ir-PRL in the tumor tissue was always associated with positive PRL mRNA, indicating production of PRL in GH-secreting tumors. Among the three patients with hyperprolactinemia, both ir-PRL and PRL mRNA was revealed in the tumor tissue of one, PRL mRNA but not ir-PRL was detected in the adenoma tissue of another, and neither PRL mRNA nor ir-PRL was found in the tumor tissue of the third. The association of hyperprolactinemia with the presence of both ir-PRL and PRL mRNA or PRL mRNA alone is indicative of PRL production and secretion. However, the absence of ir-PRL and PRL mRNA in the tumor tissue may indicate that hyperprolactinemia is caused by the suppression of PRL inhibitory factor due to hypothalamic dysfunction by the tumor mass. Thus, the study of PRL gene expression and immunohistochemistry in GH-secreting adenomas is valuable to understanding the pathophysiology of pituitary tumors.
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC
通过原位杂交分析人生长激素和催乳素分泌垂体腺瘤。
DOI：
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发表时间：
1989
期刊：
The American journal of pathology
影响因子：
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作者：
Lloyd,RV;Cano,M;Chandler,WF;Barkan,AL;Horvath,E;Kovacs,K
通讯作者：
Kovacs,K