Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells.
Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells.
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在哺乳动物上皮细胞中钙粘着蛋白细胞接触的形态发生过程中，肌球蛋白VI和VINCULIN合作。
DOI：
10.1083/jcb.200612042
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发表时间：
2007-07-30
期刊：
影响因子：
--
通讯作者：
Yap AS
中科院分区：
文献类型：
--
作者：
Maddugoda MP;Crampton MS;Shewan AM;Yap AS
钙粘蛋白和肌动蛋白细胞骨架之间的合作控制上皮生物发生的许多方面。我们在这里报道肌球蛋白 VI 严格调节上皮细胞-细胞接触的形态发生。随着上皮单层在培养中成熟，不连续的细胞与细胞接触最初被连续（粘性）接触所取代。当细胞变得线性和粘性时，肌球蛋白 VI 被募集到细胞接触处，与上皮钙粘蛋白 (E-钙粘蛋白) 形成生化复合物。肌球蛋白 VI 对于强钙粘蛋白粘附、细胞形成粘性线性接触以及顶端连接复合体的完整性是必需的。我们发现纽蛋白介导肌球蛋白 VI 的这种作用。肌球蛋白 VI 是纽蛋白和 E-钙粘蛋白相互作用所必需的。功能获得和丧失方法的结合将纽蛋白确定为肌球蛋白 VI 的下游效应子，对于细胞间接触的完整性是必需的。我们提出肌球蛋白 VI 和纽蛋白形成一种分子装置，在培养的哺乳动物上皮细胞中产生粘性细胞-细胞接触。
Cooperation between cadherins and the actin cytoskeleton controls many aspects of epithelial biogenesis. We report here that myosin VI critically regulates the morphogenesis of epithelial cell–cell contacts. As epithelial monolayers mature in culture, discontinuous cell–cell contacts are initially replaced by continuous (cohesive) contacts. Myosin VI is recruited to cell contacts as they become linear and cohesive, where it forms a biochemical complex with epithelial cadherin (E-cadherin). Myosin VI is necessary for strong cadherin adhesion, for cells to form cohesive linear contacts, and for the integrity of the apical junctional complex. We find that vinculin mediates this effect of myosin VI. Myosin VI is necessary for vinculin and E-cadherin to interact. A combination of gain and loss of function approaches identifies vinculin as a downstream effector of myosin VI that is necessary for the integrity of intercellular contacts. We propose that myosin VI and vinculin form a molecular apparatus that generates cohesive cell–cell contacts in cultured mammalian epithelia.