GERM CELL-LESS ENCODES A CELL-TYPE-SPECIFIC NUCLEAR PORE-ASSOCIATED PROTEIN AND FUNCTIONS EARLY IN THE GERM-CELL SPECIFICATION PATHWAY OF DROSOPHILA
GERM CELL-LESS ENCODES A CELL-TYPE-SPECIFIC NUCLEAR PORE-ASSOCIATED PROTEIN AND FUNCTIONS EARLY IN THE GERM-CELL SPECIFICATION PATHWAY OF DROSOPHILA
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DOI：
10.1101/gad.8.18.2123
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发表时间：
1994-09-15
影响因子：
10.5
通讯作者：
JAN, YN
中科院分区：
文献类型：
--
作者：
JONGENS, TA;ACKERMAN, LD;JAN, YN
果蝇胚胎后极的母体提供的极质含有指定生殖细胞前体（极细胞）和后轴的决定因素。一种极浆成分，即无生殖细胞基因的产物，已被发现是极细胞规格所必需的，但不是后体细胞。 gcl水平降低的母亲所生出的后代缺乏极细胞，但其他方面均正常。另一方面，过度表达 gcl 的母亲产生的后代表现出极细胞的短暂增加。 gcl 异位定位到胚胎的前极，导致该位置的细胞核适应极细胞核的特征，同时体细胞损失。我们还提供了证据表明 gcl 蛋白与极细胞核的核孔特异性相关。这种定位表明了生殖系细胞命运规范的一种新机制。
The maternally supplied pole plasm at the posterior pole of a Drosophila embryo contains determinants that specify both the germ-cell precursors (pole cells) and the posterior axis. One pole plasm component, the product of the germ cell-less gene, has been found to be required for specification of pole cells, but not posterior somatic cells. Mothers with reduced levels of gcl give rise to progeny that lack pole cells, but are otherwise normal. Mothers overexpressing gcl, on the other hand, produce progeny exhibiting a transient increase of pole cells. Ectopic localization of gcl to the anterior pole of the embryo causes nuclei at that location to adapt characteristics of pole cell nuclei, with concurrent loss of somatic cells. We also present evidence indicating that the gcl protein associates specifically with the nuclear pores of the pole cell nuclei. This localization suggests a novel mechanism in the specification of cell fate for the germ line.