Neutral red retention by lysosomes from earthworm (Lumbricus rubellus) coelomocytes: A simple biomarker of exposure to soil copper
Neutral red retention by lysosomes from earthworm (Lumbricus rubellus) coelomocytes: A simple biomarker of exposure to soil copper
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DOI：
10.1002/etc.5620151022
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发表时间：
1996-10
影响因子：
4.1
通讯作者：
J. Weeks;C. Svendsen
中科院分区：
文献类型：
--
作者：
J. Weeks;C. Svendsen
一种简单的亚细胞组织化学染色技术，采用溶酶体探针中性红已开发用于与epiendogeic红蚯蚓Hoffmeister。将从蚯蚓体腔中提取的体腔细胞放入等渗缓冲液中，使其粘附在显微镜载玻片上30秒，然后应用中性红染料。这种红色染料在溶酶体内迅速积累。这种染料从这些溶酶体到周围的胞质溶胶的损失的观察，使量化的程度造成的溶酶体损害蚯蚓暴露于土壤铜浓度的增加，在实验室和围隔研究。这种简单的体外生物标记物具有快速评估重金属和类金属污染土壤对蚯蚓毒性效应的潜力。
A simple subcellular histochemical staining technique employing the lysosomal probe neutral red has been developed for use with the epiendogeic earthworm Lumbricus rubellus Hoffmeister. Coelomocytes extracted from the coelomic cavity of earthworms into an isotonic earthworm Ringer solution were allowed to adhere to a microscope slide for 30 s before the application of a neutral red dye. This red dye was rapidly accumulated within the lysosomes. Observation of the loss of this dye from these lysosomes into the surrounding cytosol has enabled the quantification of the degree of lysosomal damage caused to earthworms with exposure to an increasing range of soil copper concentrations, in both laboratory and mesocosm studies. This simple in vitro biomarker has potential for the rapid assessment of the toxic effects to earthworms from soils contaminated with heavy metals and metalloids.