N-halamine-based multilayers on titanium substrates for antibacterial application
N-halamine-based multilayers on titanium substrates for antibacterial application
复制标题
钛基材上基于 N-卤胺的多层膜的抗菌应用
DOI：
10.1016/j.colsurfb.2018.06.039
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Cai Kaiyong
中科院分区：
文献类型：
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作者：
Tao Bailong;Shen Xinkun;Yuan Zhang;Ran Qichun;Shen Tingting;Pei Yuxia;Liu Ju;He Ye;Hu Yan;Cai Kaiyong
Bacterial infection is one of the most severe postoperative complications leading to clinical orthopedic implants failure. To improve the antibacterial property of titanium (Ti) substrates, a bioactive coating composed of chitosan-1-(hydroxymethyl)- 5,5-dimethylhydantoin (Chi–HDH-Cl) and gelatin (Gel) was fabricated via layer-by-layer (LBL) assembly technique. The results of Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1HNMR) and X-ray photoelectron spectroscopy (XPS) showed that Chi−HHD-Cl conjugate was successfully synthesized. Scanning electron microscopy (SEM), atomic force microscope (AFM) and water contact angle measurements were employed to monitor the morphology, roughness changes and surface wettability of Ti substrates, which proved the multilayers coating formation. Antibacterial assay againstEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus) revealed that the Gel/Chi–HDH-Cl modified Ti substrates most efficiently inhibited the adhesion and growth of bacteria. Meanwhile,in vitrocellular tests confirmed that Gel/Chi–HDH-Cl multilayers had no obvious cytotoxicity to osteoblasts. The study thus provides a promising method to fabricate antibacterial Ti-based substrates for potential orthopedic application.
