The Vacuole and Mitochondria Patch (vCLAMP) Protein Vam6 is Crucial for Autophagy in Candida albicans
The Vacuole and Mitochondria Patch (vCLAMP) Protein Vam6 is Crucial for Autophagy in Candida albicans
复制标题
液泡和线粒体补丁 (vCLAMP) 蛋白 Vam6 对于白色念珠菌的自噬至关重要
DOI：
10.1007/s11046-021-00565-x
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发表时间：
2021-05-31
期刊：
影响因子：
5.5
通讯作者：
Li, Mingchun
中科院分区：
文献类型：
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作者：
Mao, Xiaolong;Yang, Li;Li, Mingchun
酵母细胞中的线粒体膜蛋白（vCLAMPs）参与了酵母细胞的抗应激反应，但它们在自噬中的确切作用至今仍不清楚。本研究首次研究了vCLAMP核心蛋白Vam 6在白色念珠菌自噬中的作用。实验表明，VAM 6的缺失导致氮饥饿下的生长缺陷。此外，蛋白质印迹显示vam 6 Delta/Delta突变体减弱了Atg 8（自噬指示剂）、Lap 41（细胞质至液泡靶向途径的指示剂）和Csp 37（线粒体自噬指示剂）的降解。此外，羧肽酶Y的活性和空泡磷脂酶Atg 15的水平显着下降的突变体，这证实了自噬缺陷所造成的缺失VAM 6。总的来说，这些结果表明，Vam 6在维持氮饥饿下的自噬过程中是必不可少的，这为vCLAMPs和自噬之间的相关性提供了新的见解。
Vacuole and mitochondria patches (vCLAMPs) are involved in the stress resistance of yeast, but their exact role in autophagy remains so far unclear. This study, for the first time, investigated the role of the vCLAMP core protein Vam6 in autophagy of Candida albicans. The experiments demonstrated that the deletion of VAM6 led to a growth defect under nitrogen starvation. Also, western blotting revealed that the vam6 Delta/Delta mutant attenuated degradation of Atg8 (an autophagy indicator), Lap41 (an indicator of the cytoplasm to vacuole targeting pathway), and Csp37 (a mitophagy indicator). Moreover, the activity of carboxypeptidase Y and the levels of the vacuolar phospholipase Atg15 were significantly decreased in the mutant, which confirmed the defect of autophagy caused by deletion of VAM6. Overall, these results revealed that Vam6 is essential in maintaining the autophagic process under nitrogen starvation, and this provided new insights into the correlation between vCLAMPs and autophagy.