Optimization of polyhydroxybutyrate (PHB) production by excess activated sludge and microbial community analysis
Optimization of polyhydroxybutyrate (PHB) production by excess activated sludge and microbial community analysis
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通过剩余活性污泥和微生物群落分析优化聚羟基丁酸酯（PHB）生产
DOI：
10.1016/j.jhazmat.2010.08.003
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发表时间：
2011-01-15
影响因子：
13.6
通讯作者：
Li, Qingbiao
中科院分区：
文献类型：
--
作者：
Liu, Zhenggui;Wang, Yuanpeng;Li, Qingbiao
本研究利用剩余活性污泥生产高附加值、可生物降解的热塑性塑料聚羟基丁酸酯（PHB）。研究了营养条件、曝气方式、乙酸钠浓度和初始pH值对活性污泥中聚羟基丁酸（PHB）积累的影响。在初始pH值为7.0、乙酸钠浓度为6.0 g L-1、间歇曝气的条件下，该污泥的PHB含量和产率最高，分别为67.0%（菌体干重）和0.740 gCOD gCOD（-1）（COD：化学需氧量）。聚合酶链反应（PCR）-变性梯度凝胶电泳（DGGE）测序分析表明，在聚羟基丁酸（PHB）积累过程中，污泥微生物群落结构发生了显著变化。在不同的运行条件下，剩余活性污泥中发现了3株聚羟基丁酸菌，分别属于Thauera、Dechloromonas和Competibacter。(C)2010爱思唯尔有限公司版权所有。
In this study, a high value-added and biodegradable thermoplastic, polyhydroxybutyrate (PHB), was produced by excess activated sludge. The effects of the nutritional condition, aeration mode, sodium acetate concentration and initial pH value on PHB accumulation in the activated sludge were investigated. The maximum PHB content and PHB yield of 67.0% (dry cell weight) and 0.740 gCOD gCOD(-1) (COD: chemical oxygen demand), respectively, were attained by the sludge in the presence of 6.0 g L-1 sodium acetate, with an initial pH value of 7.0 and intermittent aeration. The analysis of the polymerase chain reaction (PCR)-denaturing gradient-gel-electrophoresis (DGGE) sequencing indicated that the microbial community of the sludge was significantly different during the process of PHB accumulation. Three PHB-accumulating microorganisms, which were affiliated with the Thauera, Dechloromonas and Competibacter lineages, were found in the excess activated sludge under different operating conditions for PHB accumulation. (C) 2010 Elsevier B.V. All rights reserved.