Samp1 is a component of TAN lines and is required for nuclear movement
Samp1 is a component of TAN lines and is required for nuclear movement
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DOI：
10.1242/jcs.087049
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发表时间：
2012-03-01
影响因子：
4
通讯作者：
Gomes, Edgar R.
中科院分区：
文献类型：
--
作者：
Borrego-Pinto, Joana;Jegou, Thibaud;Gomes, Edgar R.
细胞核的位置在不同的发育阶段和细胞事件中受到调节。在极化过程中，细胞核远离未来的前沿，这种运动是正确的细胞迁移所必需的。核运动需要LINC复合物组分nesprin-2G和SUN 2，它们在核膜处形成跨膜肌动蛋白相关核（TAN）线。在这里，我们表明，核包膜蛋白Samp 1（NET 5）参与成纤维细胞极化和迁移过程中的核运动。此外，我们证明，Samp 1是一个组件的TAN线，包含nesprin-2G和SUN 2。最后，Samp 1与SUN 2和核纤层蛋白A/C结合，并且Samp 1在核膜上的存在需要核纤层蛋白A/C。这些结果支持Samp 1在核运动过程中LINC复合物和核纤层蛋白之间的关联中的作用。
The position of the nucleus is regulated in different developmental stages and cellular events. During polarization, the nucleus moves away from the future leading edge and this movement is required for proper cell migration. Nuclear movement requires the LINC complex components nesprin-2G and SUN2, which form transmembrane actin-associated nuclear (TAN) lines at the nuclear envelope. Here we show that the nuclear envelope protein Samp1 (NET5) is involved in nuclear movement during fibroblast polarization and migration. Moreover, we demonstrate that Samp1 is a component of TAN lines that contain nesprin-2G and SUN2. Finally, Samp1 associates with SUN2 and lamin A/C, and the presence of Samp1 at the nuclear envelope requires lamin A/C. These results support a role for Samp1 in the association between the LINC complex and lamins during nuclear movement.