Three-dimensional porous composite scaffolds for in-vitro marrow microenvironment simulation to screen leukemia drug
Three-dimensional porous composite scaffolds for in-vitro marrow microenvironment simulation to screen leukemia drug
复制标题
三维多孔复合支架用于体外骨髓微环境模拟筛选白血病药物
DOI：
10.1088/1748-605x/ab74e2
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发表时间：
2020
影响因子：
4
通讯作者：
Yun-Ze Long
中科院分区：
文献类型：
--
作者：
Zhiyu Shang;Hao Sun;Hongfei Xiang;Jun Zhang;Miao Yu;Huaisong Zhao;Chunting Zhao;Yun-Ze Long
传统的二维培养基用于模拟白血病细胞的体外微环境，通常会导致95%的药物试验结果与后续的临床结果不同。与这种2D培养不同，3D支架更类似于骨髓微环境，因此可以更好地模拟药物对白血病细胞的作用，这有助于临床药物的初步筛选。为此，采用冷冻干燥法制备了氧化石墨烯/丝素蛋白/羧甲基壳聚糖（GO/SF/CMCS）三维支架。实验结果表明，由于GO的包埋，这些三维支架表现出更好的溶胀率。支架亲水性的改善促进了白血病细胞的粘附和增殖。与传统的2D培养相比，这种3D培养中的白血病细胞表现出更强的耐药性，这与之前报道的临床结果一致。这意味着这些三维GO/SF/CMCS支架可以很好地模拟体内骨髓微环境，使其成为一个有前途的平台，初步药物筛选的临床应用。
The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results. Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use. For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS). Experimental results show that these 3D scaffolds exhibit a better swelling ratio because of the embedding of GO. The improved hydrophilicity of the scaffolds brings about promoted adhesion and proliferation of leukemia cells. In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results. It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.