DIgR1, a novel membrane receptor of the immunoglobulin gene superfamily, is preferentially expressed by antigen-presenting cells
DIgR1, a novel membrane receptor of the immunoglobulin gene superfamily, is preferentially expressed by antigen-presenting cells
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DOI：
10.1006/bbrc.2001.5539
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发表时间：
2001-09-14
影响因子：
3.1
通讯作者：
Cao, XT
中科院分区：
文献类型：
--
作者：
Luo, K;Zhang, WP;Cao, XT
免疫球蛋白基因超家族（IgSF）的一种新的膜受体已从小鼠树突状细胞（DC）中鉴定出来，命名为DC衍生的Ig样受体1（DIgR 1）。它编码228个氨基酸残基的多肽，具有21个氨基酸的信号肽、20个氨基酸的跨膜区、189个氨基酸的胞外区和19个氨基酸的胞内区。它的胞外区含有一个单一的IG V结构域。因此，它是一种新的IgSF I型跨膜糖蛋白。DIgR 1显示与人CMRF-35抗原和多聚免疫球蛋白受体（pIgR）的显著同源性。DIgR 1在小鼠脾脏中的mRNA表达量较高。在已知的抗原呈递细胞（APC）（包括DC、单核细胞/巨噬细胞和B淋巴细胞）中观察到DIgR 1 mRNA的优先表达。用抗DIgR 1多克隆抗体通过Western印迹分析检测用DIgR 1 cDNA转染的NIH/3 T3细胞中的40 kDa蛋白。DC上DIgR 1蛋白的表达不受LPS刺激的调节。DIgR 1在APC免疫生物学中的作用有待进一步研究。(C)北京：科学出版社.
A novel membrane receptor of immunoglobulin gene superfamily (IgSF) has been identified from mouse dendritic cells (DC) and designated as DC-derived Ig-like receptor 1 (DIgR1). It encodes a 228-amino-acid (aa) residue polypeptide with a 21-aa signal peptide, a 20-aa transmembrane region, a 189-aa extracellular region, and a 19 aa intracellular region. Its extracellular region contains a single V domain of Ig. So it is a novel type I transmembrane glycoprotein of IgSF. DIgR1 shows significant homologies to human CMRF-35 antigens and polymeric immunoglobulin receptors (pIgR). The mRNA expression of DIgR1 was highly abundant in mouse spleen. The preferential expression of DIgR1 mRNA is observed in the known antigen-presenting cells (APC) including DC, monocytes/macrophages, and B lymphocytes. A 40 kDa of protein in NIH/3T3 cells transfected with the DIgR1 cDNA was detected by Western blot analysis using anti-DIgR1 polyclonal antibodies. The expression of DIgR1 protein on DC is not regulated by LPS stimulation. Further study should be conduct to investigate what were biological functions of DIgR1 in the immunobiology of APC. (C) 2001 Academic Press.