Hydration properties of symmetric chain and asymmetric chain sphingomyelin bilayers
Hydration properties of symmetric chain and asymmetric chain sphingomyelin bilayers
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DOI：
10.1007/s10973-006-7659-2
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发表时间：
2006-08
影响因子：
4.4
通讯作者：
Y. Kawasaki;A. Kuboki;S. Ohira;M. Kodama
中科院分区：
文献类型：
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作者：
Y. Kawasaki;A. Kuboki;S. Ohira;M. Kodama
比较了对称链鞘磷脂(N-Palmitoylsphingmarelin)和不对称链鞘磷脂(N-lunoceroylsphingmarelin)两种脂双层体系的水化性能。以天然鞘磷脂为起始原料，通过脱酰化-反应法合成了这些神经鞘磷脂。通过对差示扫描量热仪(DSC)获得的融冰曲线进行去卷积分析，估计了不同结合态水分子的数量，并用其构建了这些水分子的水分布图。与用于比较的天然鞘磷脂类似，不对称链鞘磷脂被发现形成具有内腔的小囊泡，并将每个脂质分子中的15个水分子结合到其腔内，相比之下，由对称链鞘磷脂形成的大尺寸多层囊泡的冻结层间水为5H2O/脂质。
Hydration properties of lipid bilayer systems are compared for symmetric chain sphingomyelin (N-palmitoylsphingomyelin) and asymmetric chain sphingomyelin (N-lignoceroylsphingomyelin). These sphingomyelins were semisynthesized by a deacylation- reacylation process with a natural sphingomyelin used as a starting material. The number of differently bound water molecules was estimated by a deconvolution analysis of the ice-melting curves obtained by a differential scanning calorimetry (DSC) and was used to construct a water distribution diagram for these water molecules. Similarly to a natural sphingomyelin used for comparison, the asymmetric chain sphingomyelin was found to form small size vesicles having an internal cavity and incorporate 15 water molecules per molecule of lipid into its cavity, in contrast with 5 H2O/lipid for freezable interlamellar water observed for large size multilamellar vesicles formed by the symmetric chain sphingomyelin.