The Diversity and Ubiquity of Antibiotic Resistance Genes in Finfish Culture Ponds in Bangladesh
The Diversity and Ubiquity of Antibiotic Resistance Genes in Finfish Culture Ponds in Bangladesh
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DOI：
10.1101/2022.09.14.507951
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发表时间：
2022-09
期刊：
影响因子：
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通讯作者：
Ashley G. Bell;K. Thornber;Dominique L. Chaput;N. A. Hasan;M. Z. Alam;M. M. Haque-M.;J. Cable;B. Te
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文献类型：
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作者：
Ashley G. Bell;K. Thornber;Dominique L. Chaput;N. A. Hasan;M. Z. Alam;M. M. Haque-M.;J. Cable;B. Te
在孟加拉国，鱼类提供了60%以上的动物源食物，其中56.2%来自主要在农村淡水池塘生产的水产养殖。对鱼类产品需求的增加正在推动集约化，导致疾病患病率上升，对粮食安全构成风险。孟加拉国农村水产养殖实践中往往缺乏生物安全，抗生素通常用于治疗和预防疾病爆发。抗生素经常被错误地使用-这是与抗生素耐药性（AMR）发展相关的关键因素。AMR可以通过移动的遗传元件在微生物生态系统内迅速传播，随着抗生素治疗无效，对感染AMR病原体的人类和动物构成风险。早期AMR检测和了解农村水产养殖实践中抗生素耐药基因（ARG）的传播对于粮食安全和人类健康保护至关重要。在这里，我们应用宏基因组学的方法来评估的ARG组成的池塘水从六鳍鱼（罗非鱼和pangasius）农场在Mymensingh师孟加拉国中北部。我们发现池塘内的微生物群落具有相似的α和β双功能，预测多种ARG会对18种不同类别的抗菌剂产生耐药性。最常见的ARG赋予耐氨基糖苷类和磺胺类药物，并存在于鱼类和人类病原体相关的类群。这种ARG的多样性可能会对各种抗生素类产生耐药性，并质疑目前和未来在土制水产养殖池塘中使用抗生素治疗疾病的有效性。每个养殖场内鱼塘之间的微生物和ARG组成相似，这可能与养殖实践中的相似之处有关，从而产生相似的微生物选择压力，从而产生可比的微生物种群。如果不对抗生素的使用采取更严格的控制措施，随着孟加拉国水产养殖生产的加剧，将不可避免地进一步加剧治疗和预防疾病爆发的挑战。孟加拉国农村鱼塘中的ARG表明对18种不同抗生素具有耐药性最常见的AMR是氨基糖苷类和磺胺类ARG存在于质粒和分类群相关病原体中养殖实践强烈影响微生物和ARG组成已鉴定的ARG质疑农村水产养殖疾病的抗生素治疗
In Bangladesh, fish provide over 60% of animal-source food with 56.2% of this coming from aquaculture produced predominantly in rural freshwater ponds. Increasing demand for fish products is driving intensification and resulting in higher disease prevalence, posing a risk to food security. Biosecurity is often absent in rural aquaculture practices in Bangladesh and antibiotics are commonly used to treat and prevent disease outbreaks. Antibiotics are often administered incorrectly - a key factor associated with the development of antimicrobial resistance (AMR). AMR can be disseminated rapidly within microbial ecosystems via mobile genetic elements, posing a risk for humans and animals infected with AMR pathogens as treatments with antibiotics become ineffective. Early AMR detection and understanding of the spread of antimicrobial resistant genes (ARGs) in rural aquaculture practices is critical for both food security and human health protection. Here, we apply a metagenomic approach to assess the ARG composition in pond water from six finfish (tilapia and pangasius) farms in the Mymensingh division of North-central Bangladesh. We found microbial communities within the ponds had similar alpha and beta diversities, with multiple ARGs predicted to confer resistance to eighteen different classes of antimicrobials. The most common ARGs conferred resistance to aminoglycosides and sulphonamides and were present in taxa associated with both fish and human pathogens. This ARG diversity potentially confers resistance to a wide variety of antibiotic classes and questions the effectiveness of current and future treatment of diseases with antibiotics in earthen aquaculture ponds. The microbial and ARG compositions between fish ponds within each farm were similar, which may relate to parallels in farming practices creating similar microbial selection pressures and thus comparable microbial populations. Without a more controlled approach towards antibiotic usage, will inevitably further exacerbate the challenges in treating and preventing disease outbreaks as aquaculture production intensifies in Bangladesh. Highlights ARGs in Bangladesh rural fishponds indicate resistance to 18 different antibiotics The most common AMR were to aminoglycosides and sulphonamides ARGs were present in plasmids and taxa-associated pathogens Farming practices strongly influence microbial and ARG compositions Identified ARGs question antibiotic treatment of disease in rural aquaculture