MITOCHONDRIAL DYSFUNCTION IS ASSOCIATED WITH AN IMMUNE PARALYSIS PHENOTYPE IN PEDIATRIC SEPSIS
MITOCHONDRIAL DYSFUNCTION IS ASSOCIATED WITH AN IMMUNE PARALYSIS PHENOTYPE IN PEDIATRIC SEPSIS
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DOI：
10.1097/shk.0000000000001486
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发表时间：
2020-09-01
期刊：
影响因子：
3.1
通讯作者：
Wallace, Douglas C.
中科院分区：
文献类型：
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作者：
Weiss, Scott L.;Zhang, Donglan;Wallace, Douglas C.
目的：免疫功能紊乱是脓毒症的一个重要特征，但线粒体在小儿败血症免疫麻痹发生发展中的作用尚不清楚。我们试图确定外周血单核细胞(PBMCs)中检测到的线粒体功能障碍是否与脓毒症儿童的免疫麻痹和全身炎症有关。设计：前瞻性观察性研究。单位：单科儿科重症监护室(PICU)。患者：161例败血症/感染性休克儿童和18例非感染性PICU对照。检测方法和主要结果：在脓毒症识别后的第1~2天(T1)、第3~5天(T2)和第8~14天(T3)分别测定PBMC的线粒体呼吸、免疫功能标志物和血浆细胞因子，并测定对照组的1次。免疫麻痹被定义为全血体外脂多糖诱导的肿瘤坏死因子-α(TNF-α)
Objective: Immune dysregulation is a defining feature of sepsis, but the role for mitochondria in the development of immunoparalysis in pediatric sepsis is not known. We sought to determine if mitochondrial dysfunction measured in peripheral blood mononuclear cells (PBMCs) is associated with immunoparalysis and systemic inflammation in children with sepsis. Design: Prospective observational study. Setting: Single-academic pediatric intensive care unit (PICU). Patients: One hundred sixty-one children with sepsis/septic shock and 18 noninfected PICU controls. Measurements and Main Results: Mitochondrial respiration in PBMCs, markers of immune function, and plasma cytokines were measured on days 1 to 2 (T1), 3 to 5 (T2), and 8 to 14 (T3) after sepsis recognition, and once for controls. Immunoparalysis was defined as whole-blood ex vivo lipopolysaccharide-induced tumor necrosis factor-alpha (TNF-alpha)