Ultraviolet-induced accumulation of phytoalexins in rice leaves.
Ultraviolet-induced accumulation of phytoalexins in rice leaves.
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DOI：
10.1271/bbb1961.52.2469
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发表时间：
1988-10
期刊：
影响因子：
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通讯作者：
O. Kodama;Takashi Suzuki;Junichi Miyakawa;T. Akatsuka
中科院分区：
文献类型：
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作者：
O. Kodama;Takashi Suzuki;Junichi Miyakawa;T. Akatsuka
采用BOND ELUT C18固相萃取柱预处理水稻离体叶片，用气相色谱-质谱-选择离子监测法（GC-MS-SIM）检测紫外线照射后叶片中积累的植物抗毒素。观察到稻毒素A、B、C和D、momilactone A和B以及一种未知抗真菌物质的积累，并伴有叶面出现棕色斑点。检测到大量的Momilactone A，并且在oryzalexins中，oryzalexin D是主要物质。这些植物抗毒素的最大积累，除了oryzalxins C和D，被发现照射后3天。而水稻抗毒素D的最大积累量出现在照射后2d，而水稻抗毒素C的最大积累量出现在照射后4d。水稻叶片中这些二萜类植物抗毒素的含量取决于叶片的老化程度，最上部叶片中这些植物抗毒素的积累量远低于老化叶片（下部叶片），并且表面几乎不出现褐斑。
Accumulated phytoalexins in ultraviloet-irradiated detached rice leaves were detected by gas chromatography-mass spectrometry-selected ion monitoring (GC-MS-SIM) after pretreating with a BOND ELUT C18 cartridge. An accumulation of oryzalexins A, B, C and D, momilactones A and B, and an unknown antifungal substance accompanied by the appearance of brown spots on the leaf surface was observed. Momilactone A was detected in abundance, and among the oryzalexins, oryzalexin D was a major substance. The maximum accumulation of these phytoalexins, except for oryzalxins C and D, was found 3 days after irradiation. However, the maximum accumulation of oryzalexin D was observed 2 days after irradiation and that of oryzalexin C was observed 4 days after it. The content of these diterpenoid phytoalexins in rice leaves was dependent on leaf aging, an accumulation of these phytoalexins in the uppermost leaves being much lower than that in the aged leaves (lower leaves), and brown spots scarcely ever appeared on the sur...