The Origin of Stroma Surrounding Epithelial Ovarian Cancer Cells
The Origin of Stroma Surrounding Epithelial Ovarian Cancer Cells
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DOI：
10.1097/pgp.0b013e3182518533
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发表时间：
2013-01-01
影响因子：
2.4
通讯作者：
Aoki, Daisuke
中科院分区：
文献类型：
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作者：
Akahane, Tomoko;Hirasawa, Akira;Aoki, Daisuke
肿瘤间质被认为在肿瘤行为中起着重要作用，包括侵袭或转移以及对治疗的反应。癌症间质通常被认为是非肿瘤性细胞，包括组织骨髓或骨髓来源的成纤维细胞，或者起源于癌细胞的上皮间充质转化。在这项研究中，我们评估了p53基因在上皮性卵巢癌（EOC）的癌细胞和癌间质中的地位，以阐明间质的起源。本研究纳入了16例EOC患者的样本。显微解剖肿瘤细胞和邻近的非肿瘤间质细胞，分别提取DNA。我们分析了所有病例中癌症和间质组织的p53序列（外显子5-8）。此外，我们检测了所有病例中p53蛋白的表达。在16例EOCs中有9例检测到p53突变，其中8例仅在癌细胞中检测到突变。其中1例在癌组织和间质组织中均检测到相同的突变（R248Q），在癌细胞和癌间质中均检测到p53蛋白表达。EOC的大多数癌间质被认为起源于非肿瘤细胞，但部分癌间质可能起源于癌细胞。
Cancer stroma is thought to play an important role in tumor behavior, including invasion or metastasis and response to therapy. Cancer stroma is generally thought either to be non-neoplastic cells, including tissue-marrow or bone-marrow-derived fibroblasts, or to originate in epithelial mesenchymal transition of cancer cells. In this study, we evaluated the status of the p53 gene in both the cancer cells and the cancer stroma in epithelial ovarian cancer (EOC) to elucidate the origin of the stroma. Samples from 16 EOC patients were included in this study. Tumor cells and adjacent nontumor stromal cells were microdissected and DNA was extracted separately. We analyzed p53 sequences (exons 5-8) of both cancer and stromal tissues in all cases. Furthermore, we examined p53 protein expression in all cases. Mutations in p53 were detected in 9 of the 16 EOCs: in 8 of these cases, the mutations were detected only in cancer cells. In 1 case, the same mutation (R248Q) was detected in both cancer and stromal tissues, and p53 protein expression was detected in both the cancer cells and the cancer stroma. Most cancer stroma in EOC is thought to originate from non-neoplastic cells, but some parts of the cancer stroma might originate from cancer cells.