Incubation temperature and satiety influence general locomotor and exploratory behaviors in the common snapping turtle (Chelydra serpentina)
Incubation temperature and satiety influence general locomotor and exploratory behaviors in the common snapping turtle (Chelydra serpentina)
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DOI：
10.1016/j.physbeh.2020.112875
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发表时间：
2020-06-01
影响因子：
2.9
通讯作者：
Rhen, Turk
中科院分区：
文献类型：
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作者：
Miller, Soleille;Derenne, Adam;Rhen, Turk
胚胎发育期间的温度决定性别，并且已被证明会影响其他生理特征。北美拟鳄龟（Chelydra serpentina）是一个理想的模型，用于测试温度如何影响具有温度依赖性性别决定的物种的行为。行为分析对于理解气候变化如何影响拟鳄龟的整体机体功能至关重要。目前，针对像龟这样的半水生脊椎动物的行为分析很少。在这项研究中，我们在2018年使用数码相机在露天场地环境下记录了来自不同孵化温度的喂食和禁食的幼龟的行为，时长为20分钟，并在2019年重复了该实验。露天场地是圆形水池，水深3.5厘米。每个场地被分成四个象限和两个区域（内部和外部）。使用开源图像分析软件（ImageJ）从视频中自动收集并总结了龟积极移动的时间、移动的总距离以及其他一些指标。饱腹感和孵化温度对移动的总距离、移动所花费的时间以及在外部区域移动的时间有显著影响。这些发现表明，胚胎发育期间的温度对龟的探索性或一般运动行为背后的神经机制具有长期影响。
Temperature during embryogenesis determines sex and has been shown to influence other physiological traits in replies. The common snapping turtle (Chelydra serpentina) is an ideal model for testing how temperature impacts behavior in species that display temperature-dependent sex determination. Behavioral assays are crucial to understanding how a changing climate may affect whole organism function in the snapping turtle. Currently, there are few behavioral assays for semi-aquatic vertebrates like turtles. In this study, we used digital cameras to record behavior of fed and fasted hatchling turtles from different incubation temperatures in an open field setting for 20 min in 2018 and repeated the experiment in 2019. Open fields were circular tanks filled with water to a depth of 3.5 cm. Each field was split into four quadrants and two zones (inner and outer). The amount of time turtles spent actively moving, total distance travelled, and several other measures were collected and summarized automatically from videos with open source image analysis software (ImageJ). Satiety and incubation temperature had significant effects on total distance moved, time spent moving, and time moving in the outer zone. These findings indicate that temperature during embryogenesis has a long-lasting effect on neural mechanisms underlying exploratory or general locomotor behavior in turtles.