Environmental Bacteriophages of the Emerging Enterobacterial Phytopathogen, Dickeya solani, Show Genomic Conservation and Capacity for Horizontal Gene Transfer between Their Bacterial Hosts.
Environmental Bacteriophages of the Emerging Enterobacterial Phytopathogen, Dickeya solani, Show Genomic Conservation and Capacity for Horizontal Gene Transfer between Their Bacterial Hosts.
复制标题
DOI：
10.3389/fmicb.2017.01654
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Salmond GPC
中科院分区：
文献类型：
--
作者：
Day A;Ahn J;Fang X;Salmond GPC
Dickeya solani 是一种在欧洲大陆广泛传播的重要经济植物病原体，可导致马铃薯作物减产 25%。目前还没有有效的环境可接受的化学系统可用于治疗迪克雅引起的疾病。噬菌体已被建议用于该病原体的现场生物防治，并且已经进行了有限的现场试验。迄今为止，仅分离和鉴定了少量能够感染立枯丝核菌的噬菌体，因此需要扩展可能在噬菌体治疗策略中具有潜在用途的噬菌体库。在这里，我们描述了来自环境来源的 67 种噬菌体，其中大多数是肌病毒科病毒的成员。对两个分离株的全基因组测序显示，其与过去 5 年在欧洲分离的 D. solani 噬菌体具有高度的 DNA 一致性，表明该噬菌体家族具有广泛的生态分布。转导实验表明，大多数新的环境噬菌体能够促进有效的水平基因转移。在对转导噬菌体敏感的宿主之间可能存在毒力或抗生素抗性基因无意转移的风险，因此在对特定噬菌体的转导能力进行充分测试之前，不要将其环境用于生物防治。
Dickeya solani is an economically important phytopathogen widespread in mainland Europe that can reduce potato crop yields by 25%. There are no effective environmentally-acceptable chemical systems available for diseases caused by Dickeya. Bacteriophages have been suggested for use in biocontrol of this pathogen in the field, and limited field trials have been conducted. To date only a small number of bacteriophages capable of infecting D. solani have been isolated and characterized, and so there is a need to expand the repertoire of phages that may have potential utility in phage therapy strategies. Here we describe 67 bacteriophages from environmental sources, the majority of which are members of the viral family Myoviridae. Full genomic sequencing of two isolates revealed a high degree of DNA identity with D. solani bacteriophages isolated in Europe in the past 5 years, suggesting a wide ecological distribution of this phage family. Transduction experiments showed that the majority of the new environmental bacteriophages are capable of facilitating efficient horizontal gene transfer. The possible risk of unintentional transfer of virulence or antibiotic resistance genes between hosts susceptible to transducing phages cautions against their environmental use for biocontrol, until specific phages are fully tested for transduction capabilities.