Material properties of degradable alloy Fe-30Mn-0.6N and its effect on ferroptosis in synoviocytes.
Material properties of degradable alloy Fe-30Mn-0.6N and its effect on ferroptosis in synoviocytes.
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DOI：
10.1002/jbm.b.35139
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发表时间：
2022-09
期刊：
影响因子：
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通讯作者：
Tianyu Yang;Shimin Hao;Penghao Wang;Yu Qin;Guanchao You;Yunyi Shi;Boning Yang;Ao Zhang;Lei Guo;Tianlong Jiang
中科院分区：
文献类型：
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作者：
Tianyu Yang;Shimin Hao;Penghao Wang;Yu Qin;Guanchao You;Yunyi Shi;Boning Yang;Ao Zhang;Lei Guo;Tianlong Jiang
铁合金已显示出作为植入材料的潜力，但其对滑膜组织铁性下垂的影响却鲜有人关注。本研究旨在检测Fe-30Mn-0.6N合金的力学性能、降解性、生物相容性以及对滑膜组织铁性下垂的影响。拉伸试验表明，Fe-30Mn-0.6N合金的抗拉强度为487±18 Mpa，屈服强度为221±10 Mpa，延伸率为16.9±0.3%，杨氏模数为37.7±1.3 Gpa。体内实验表明，注入8周后，Fe-30Mn-0.6N合金的横截面积减小了73.32±12.73%。扫描电子显微镜(SEM)和表面元素分析(EDS)结果表明，Fe-30Mn-0.6N合金在体外有更多的Ca、O、C和P元素沉积(p.05)，细胞内Fe~(2+)和活性氧(ROS)显著减少(p<；.05)。WB和PCR结果显示，30%提取物可提高滑膜细胞Gpx4、FTH1和SLC7A11的蛋白活性和mRNA表达，而对Ptgs2和P53的表达无明显影响。结论：Fe-30Mn-0.6N在种植体周围滑膜组织中具有可降解性和生物相容性，对滑膜细胞无明显的铁性下垂。
Ferroalloy has shown potential as implant materials, but little attention has been paid to their effects on synovial tissue ferroptosis. This study aimed to examine the mechanical properties, degradability and biocompatibility of Fe-30Mn-0.6N alloy and effects of it on synovial tissue ferroptosis. Tensile testing showed that Fe-30Mn-0.6N alloys exhibited tensile strength of 487 ± 18 MPa, yield strength of 221 ± 10 MPa, elongation of 16.9 ± 0.3% and Young's modulus of 37.7 ± 1.3 GPa. In vivo experiments, the cross-sectional area of the Fe-30Mn-0.6N alloys decreased by 73.32 ± 12.73% after 8 weeks of implantation. The results of scanning electron microscopy (SEM) and surface elemental analysis (EDS) showed that the Fe-30Mn-0.6N alloys had more Ca, O, C and P element deposition (p .05) in vitro, and intracellular Fe2+ and the reactive oxygen species (ROS) was significantly reduced (p < .05). WB and PCR results showed that the 30% extracts increased the protein activity and mRNA expression of GPX4, FTH1 and SLC7A11 in synoviocytes, but had no effect on PTGS2 and p53. It is concluded that Fe-30Mn-0.6N had degradability and biocompatibility in peri-implant synovial tissue, and did not induce significantly ferroptosis in synoviocytes.