Identification of novel neural- and neural retina-specific antigens with a monoclonal antibody.
Identification of novel neural- and neural retina-specific antigens with a monoclonal antibody.
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用单克隆抗体鉴定新型神经和神经视网膜特异性抗原。
DOI：
10.1073/pnas.81.7.2260
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发表时间：
1984
影响因子：
11.1
通讯作者：
L. Glaser
中科院分区：
文献类型：
--
作者：
G. J. Cole;L. Glaser
荧光激活的细胞分选筛选方法已被用来确定杂交瘤分泌的单克隆抗体，可以结合到胚胎鸡神经视网膜细胞的可行的亚群。一种单克隆抗体，C1 H3，识别两个神经组织特异性多肽，表现出不同的发展模式。单克隆抗体与140千道尔顿（kDa）多肽反应，该多肽在发育早期（第7天）存在，但在后期（第17天）通过免疫印迹检测到的量可忽略不计。相反，170-kDa多肽在第10天首先通过免疫印迹检测到，并且在第17天是主要的C1 H3抗原。两种蛋白质的蛋白水解片段的分析表明，多肽是不同的分子，共享一个共同的抗原决定簇。这两种多肽都是神经特异性的; 140-kDa多肽似乎是视网膜特异性的，而170-kDa多肽也存在于神经系统的其他区域。在早期胚胎阶段，视网膜细胞原位的代谢标记显示仅合成140-kDa多肽。当这些细胞在体外解离和标记时，它们主要合成170-kDa多肽。因此，这两种多肽合成的差异速率受环境因素控制，所述环境因素可能包括细胞-细胞接触或未知的系统性因素。140-kDa多肽是早期神经视网膜细胞的独特标志物。
A fluorescence-activated cell sorter screening method has been used to identify hybridomas that secrete monoclonal antibodies that can bind to viable subpopulations of embryonic chicken neural retina cells. One monoclonal antibody, C1H3, recognizes two nervous tissue-specific polypeptides that exhibit distinct developmental patterns. The monoclonal antibody reacts with a 140-kilodalton (kDa) polypeptide that is present at early stages of development (day 7) but is detected by immunoblotting in only negligible amounts at later times (day 17). In contrast, a 170-kDa polypeptide is first detectable by immunoblotting at day 10 and is the predominant C1H3 antigen at day 17. Analysis of proteolytic fragments of the two proteins indicates that the polypeptides are distinct molecules that share a common antigenetic determinant. Both polypeptides are neural-specific; the 140-kDa polypeptide appears to be retina-specific, while the 170-kDa polypeptide is also present in other areas of the nervous system. Metabolic labeling of retina cells in situ at early embryonic stages reveals only the synthesis of the 140-kDa polypeptide. When such cells are dissociated and labeled in vitro, they synthesize primarily the 170-kDa polypeptide. Thus, the differential rate of synthesis of these two polypeptides is controlled by environmental factors that possibly include cell-cell contacts or an unknown systemic factor. The 140-kDa polypeptide is a unique marker for early neural retina cells.
通过单克隆抗体鉴定鸡神经视网膜细胞亚群：荧光激活细胞分选仪筛选技术。
影响因子：
2.9
作者：
Dyer,SA;Derby,MA;Cole,GJ;Glaser,L
通讯作者：
Glaser,L