Cytokine- and virus-stimulated airway smooth muscle cells produce IL-11 and other IL-6-type cytokines
Cytokine- and virus-stimulated airway smooth muscle cells produce IL-11 and other IL-6-type cytokines
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DOI：
10.1152/ajplung.1997.273.3.l648
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发表时间：
1997-09-01
影响因子：
4.9
通讯作者：
Panettieri, R
中科院分区：
文献类型：
--
作者：
Elias, JA;Wu, Y;Panettieri, R
炎症和平滑肌异常在人类气道疾病中共存，并且炎症调节气道平滑肌细胞的增殖和收缩特性。为了确定人气道平滑肌（HASM）细胞是否具有调节局部炎症过程的能力，我们在体外表征了HASM细胞的白细胞介素（IL）-6型细胞因子的产生。我们证明了HASM细胞在用IL-1或转化生长因子-β 1（TGF-β 1）刺激时是IL-11的有效生产者，并且IL-11水平通过IL-1和TGF-β 1的组合协同增加。这些研究还表明，IL-1和TGF-β 1刺激的HASM细胞产生IL-6和白血病抑制因子，但不产生制瘤素M，呼吸道合胞病毒和3型副流感病毒刺激HASM细胞产生IL-11。最后，他们证明，在所有情况下，刺激HASM细胞IL-11的加工是预先介导的，因为细胞因子和病毒诱导的IL-11蛋白产生的改变与IL-11 mRNA积累的成比例变化相关。HASM细胞是IL-II和其它IL-6型细胞因子的有效生产者，并且可能是局部气道炎症的重要调节剂。
Inflammation and smooth muscle abnormalities coexist in human airway disorders, and inflammation modulates the proliferative and contractile properties of airway smooth muscle cells. To determine whether human airway smooth muscle (HASM) cells have the ability to regulate local inflammatory processes, we characterized the interleukin (IL)-6-type cytokine production of HASM cells in vitro. We demonstrated that HASM cells are potent producers of IL-11 when stimulated with IL-1 or transforming growth factor-beta 1 (TGF-beta 1) and that IL-11 levels are synergistically increased by IL-1 and TGF-beta 1 in combination. These studies also demonstrated that IL-l and TGF-beta 1-stimulated HASM cells produce IL-6 and leukemia inhibitory factor but not oncostatin M and that respiratory syncytial virus and parainfluenza virus type 3 stimulate HASM cell IL-11 production. Finally, they demonstrate that, in all cases, the stimulation of HASM cell IL-11 elaboration is pretranslationally mediated since cytokine- and virus-induced alterations in IL-11 protein production were associated with proportionate changes in IL-11 mRNA accumulation. HASM cells are potent producers of IL-ll and other IL-6-type cytokines and may be important regulators of local airway inflammation.