Controlling the Cell Adhesion Property of Silk Films by Graft Polymerization
Controlling the Cell Adhesion Property of Silk Films by Graft Polymerization
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DOI：
10.1021/am4060595
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发表时间：
2014-04-09
影响因子：
9.5
通讯作者：
Singh, Neetu
中科院分区：
文献类型：
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作者：
Dhyani, Vartika;Singh, Neetu
本文报道了一种接枝聚合方法来提高桑蚕丝素膜的细胞粘附性能。B。由于其生物相容性和生物可降解性，桑蚕丝已发展成为一种有前途的组织工程材料。然而，丝的疏水特性使得细胞粘附和增殖困难。此外，缺乏足够的反应性氨基酸残基使得通过化学修饰的生物功能化具有挑战性。我们的研究描述了一种简单的方法，该方法提供了增加的化学处理，用于调节再生丝膜（SF）的表面化学，从而允许操纵其生物活性。通过等离子体蚀刻接枝pAAc和pHEMA，我们分别增加了蚕丝上的羧酸和羟基。这些修饰使我们能够调节SF的亲水性并为生物缀合提供官能团。我们的策略还允许我们开发基于丝的表面涂层，其中可以实现对细胞粘附的空间控制。这种对SF的特定区域中的细胞粘附的控制难以通过改性丝纤蛋白而不是SF表面的现有方法获得。因此，我们的策略将是一个有价值的除了工具包的生物功能化，以提高SFs的组织工程应用。
We report here a graft polymerization method to improve the cell adhesion property of Bombyx mori silk fibroin films. B. mori silk has evolved as a promising material for tissue engineering because of its biocompatibility and biodegradability. However, silk's hydrophobic character makes cell adhesion and proliferation difficult. Also, the lack of sufficient reactive amino acid residues makes biofunctionalization via chemical modification challenging. Our study describes a simple method that provides increased chemical handles for tuning of the surface chemistry of regenerated silk films (SFs), thus allowing manipulation of their bioactivity. By grafting pAAc and pHEMA via plasma etching, we have increased carboxylic acid and hydroxyl groups on silk, respectively. These modifications allowed us to tune the hydrophilicity of SFs and provide functional groups for bioconjugation. Our strategy also allowed us to develop silk-based surface coatings, where spatial control over cell adhesion can be achieved. This control over cell adhesion in a particular region of the SFs is difficult to obtain via existing methods of modifying the silk fibroin instead of the SF surface. Thus, our strategy will be a valuable addition to the toolkit of biofunctionalization for enhancing SFs' tissue engineering applications.