Metallofluorescent Nanoparticles for Multimodal Applications.

Metallofluorescent Nanoparticles for Multimodal Applications.
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DOI:
10.1021/acsomega.7b01984
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发表时间:
2018-01-31
期刊:
影响因子:
4.1
通讯作者:
Orte A
Orte A
中科院分区:
化学3区
文献类型:
--
作者:
Delgado-Gonzalez A;Garcia-Fernandez E;Valero T;Cano-Cortes MV;Ruedas-Rama MJ;Unciti-Broceta A;Sanchez-Martin RM;Diaz-Mochon JJ;Orte A

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Herein, we describe the synthesis and application of cross-linked polystyrene-based dual-function nano- and microparticles containing both fluorescent tags and metals. Despite containing a single dye, these particles exhibit a characteristic dual-band fluorescence emission. Moreover, these particles can be combined with different metal ions to obtain hybrid metallofluorescent particles. We demonstrate that these particles are easily nanofected into living cells, allowing them to be used for effective fingerprinting in multimodal fluorescence-based and mass spectrometry-based flow cytometry experiments. Likewise, the in situ reductions of the metal ions enable other potential uses of the particles as heterogeneous catalysts.
DOI: 10.1038/nprot.2012.052
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