Single-cell RNA-Seq analysis of diabetic wound macrophages in STZ-induced mice.
Single-cell RNA-Seq analysis of diabetic wound macrophages in STZ-induced mice.
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DOI：
10.1007/s12079-022-00707-w
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发表时间：
2023-03
影响因子：
4.1
通讯作者：
Yin, Siyuan
中科院分区：
文献类型：
--
作者：
Ma, Jiaxu;Song, Ru;Liu, Chunyan;Cao, Guoqi;Zhang, Guang;Wu, Zhenjie;Zhang, Huayu;Sun, Rui;Chen, Aoyu;Wang, Yibing;Yin, Siyuan
巨噬细胞在慢性糖尿病伤口愈合中的关键作用众所周知，但之前的巨噬细胞体外分类和标记基因可能并不完全适用于慢性伤口微环境中的细胞。在慢性伤口模型中，在单细胞水平上对巨噬细胞的异质性进行了研究和分类。我们对伤口边缘内的CD45+免疫细胞进行了单细胞测序，获得了17个细胞簇，其中包括4个巨噬细胞簇。其中一个簇是先前未描述的具有破骨细胞基因表达的巨噬细胞群，对差异基因的分析揭示了其可能的功能。我们还分析了不同采样时间对照组和糖尿病伤口组巨噬细胞组之间基因表达的差异。我们描述了单核巨噬细胞的分化谱，为糖尿病慢性伤口免疫相关机制的研究提供了重要参考。在线版本包含可在 10.1007/s12079-022-00707-w 获取的补充材料。
The crucial role of macrophages in the healing of chronic diabetic wounds is widely known, but previous in vitro classification and marker genes of macrophages may not be fully applicable to cells in the microenvironment of chronic wounds. The heterogeneity of macrophages was studied and classified at the single-cell level in a chronic wound model. We performed single-cell sequencing of CD45 + immune cells within the wound edge and obtained 17 clusters of cells, including 4 clusters of macrophages. One of these clusters is a previously undescribed population of macrophages possessing osteoclast gene expression, for which analysis of differential genes revealed possible functions. We also analysed the differences in gene expression between groups of macrophages in the control and diabetic wound groups at different sampling times. We described the differentiation profile of mononuclear macrophages, which has provided an important reference for the study of immune-related mechanisms in diabetic chronic wounds. The online version contains supplementary material available at 10.1007/s12079-022-00707-w.
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DOI：
10.1161/atvbaha.117.309271
发表时间：
2017-08
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Beckers CML;Simpson KR;Griffin KJ;Brown JM;Cheah LT;Smith KA;Vacher J;Cordell PA;Kearney MT;Grant PJ;Pease RJ
通讯作者：
Pease RJ
影响因子：
2.9
作者：
Diegelmann, RF
通讯作者：
Diegelmann, RF
影响因子：
4.4
作者：
Barbaroux, Jean-Baptiste O.;Beleut, Manfred;Groves, Richard W.
通讯作者：
Groves, Richard W.
影响因子：
6.5
作者：
Florin, Lore;Knebel, Julia;Angel, Peter
通讯作者：
Angel, Peter
影响因子：
2.9
作者：
Armstrong, David G.;Swerdlow, Mark A.;Bus, Sicco A.
通讯作者：
Bus, Sicco A.